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20 protocols using afg3102

1

Focused Ultrasound Stimulation of Earthworms

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A mono-element spherically focused piezoelectric transducer (PZ28, Ferroperm, Kvistgaard, Denmark) presenting a central frequency of 1.1 MHz and a radius of curvature of 50 mm was used. The driving signal was built from two function generators (AFG1062 and AFG3102, Tektronix, France) and amplified by a 50 dB amplifier. The main ultrasound sequences used in this study consisted of a series of pulsed ultrasound bursts (f = 1.1 MHz, Ncycles = 175, PRF = 125 Hz, Npulses = 20). The waveform of the electric driving signal was controlled with an oscilloscope (Picoscope 3000 Series, Pico Technology Ltd., UK). Acoustic pressure at the focal spot was calibrated with an optical hydrophone (FOPH 2000, RP Acoustics e.K., Germany). The levels of pressure amplitudes applied with LEUS exposures ranged from 2.5 to 7.3 MPa in degassed water. The ultrasound device was placed in the bottom of a tank filled with degassed water in such a way that the focal point of the transducers was included in the surface plan of the water (Fig. 7). The earthworm was laid out and pinned with the electrodes on a Styrofoam platform held above the water, on its ventral side, a medial portion of its body, or alternatively one of its ends, being immerged 1 to 3 mm under the surface of the water.
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2

Transparent Gold Electrode Microfluidics

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Thickness of the electrodes is about 25 nm, which maintains their transparent nature and allows observation of biological cells between the electrode pairs under a microscope. Most previous studies used ITO as transparent electrodes. However, resistivity of ITO (3.0–160×10−4 Ω.cm [47 ]) is higher than gold (2.04×10−5 Ω.cm [48 ]). Transparency of thin gold electrodes combined with its low resistivity, bio-compatibility [49 (link)], and chemical inertness led us to select gold as the electrode material. In order to image the biological cells, microfluidic device is placed on an inverted microscope stage (Olympus IX81). The inlet port is connected to a syringe pump to feed the microfluidic device with 1% yeast cell suspension (88×106 cells/ml) at 1ml/hr flow rate, and outlet port is connected to a drain. Electrical ports are connected to a function generator (Tektronix AFG3102) which is programmed to apply AC signals with a desired amplitude and frequency for DEP assisted loading and unloading of the micro-wells. After capturing the cells, the electrical ports are connected to high and low terminals of a high precision impedance analyzer (HP Agilent 4194A) through a test fixture (HP 16047A). Impedance measurements are performed in 1 kHz–40 MHz frequency range, and the measured data is recorded using MATLAB R2014b software.
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3

Precise EGG Data Acquisition

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The data acquisition system consists of a precise voltage amplifier and a data acquisition card: the four-channel low-drift differential amplifier with a 20 Hz low-pass filter at -3 dB in log magnitude, and the National Instrument TM USB-6229 BNC data acquisition (DAQ) card. The amplification factor of the amplifier is calibrated by the Tektronix TM AFG3102 dual channel arbitrary/function generator and set to about 200. The DAQ card is set to an input range of ±5 V with sampling rates of 100 Hz (for experiments of dogs and human Group No. 1) and 14 Hz (for experiments of human Group No. 2). Because of a 16-bit resolution of the DAQ card, this input range corresponds to about 0.15 mV and will be suitable for the EGG acquisition.
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4

Piezoelectric and Pyroelectric OFET Characterization

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In order to induce piezoelectricity and pyroelectricity of microstructured P(VDF-TrFE) which responds to pressure and temperature, an on-chip poling process was performed by grounding source (S) and drain (D) electrodes while gate electrode is applied at −100 V.[36] Applying the pressure to the microstructured OFET was conducted by using a cyclic pushing system with a load cell while the device is positioned on a heating block with a temperature controller and a thermocouple. A two-channel arbitrary/function generator (Tektronix AFG 3102) was explored to apply sinusoidal AC VG with a frequency of 0.3125 Hz and amplitude of 20 V. We measured the device characteristics using an HP 4145B semiconductor parameter analyser with time interval of 0.01 s and −20 V of VD. The amplitude and mean values of ID induced by AC gate bias were extracted using a fast-Fourier-transform (FFT) method. These processes were also used in stimuli detection from a finger and a blunt stick by positioning the devices on the heating block.
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5

Characterization of Ferroelectric Tunnel Junctions

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40 × 40 μm2 Co/PTO/LSMO tunnel junction devices have been electrically characterized by measuring current–voltage (IV) characteristics using Keithley 2635 source-measuring units and an HTTP4 Lake-Shore cryogenic probing station. To switch the polarization orientation of the barrier, 0.5 ms wide positive/negative voltage pulses of 3 V were applied by using a Tektronix AFG 3102 function generator.
Typical IV characteristics are shown in Supplementary Fig. 5 showing the TER effect. For the 3-u.c. PTO layer, no change in current is observed, showing that the polarization cannot be switched. TMR was evaluated from measuring the tunnelling current with a 200 mV applied bias. Supplementary Fig. 3 shows a change in resistance caused by the magnetization switching in the Co and LSMO electrodes. Macroscopic magnetic hystereses measured on similar, unpatterned samples show that the coercive fields of the patterned devices are very similar to the unpatterned electrodes.
The total magnetic moment of the heterostructure (with the total area 5 × 5 mm2) was measured using an Oxford Instruments MagLab vibrating sample magnetometer in the as grown state. All measurements were performed after cooling the samples to 10 K in a −0.8 T magnetic field. TMR was calculated as where R↓↑ and R↑↑ are the resistances of the devices with antiparallel and parallel magnetizations of the electrodes.
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6

Optical and Electrical Characterization of MoTe2 Thin Films

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The optical images of the devices were taken by an optical microscope (ZEISS, Axio Imager A2m). The Raman spectra were collected by a micro-zone confocal Raman system (WITec alpha 300R) under 532 nm laser illumination. The thickness of the MoTe2 film was measured by an atomic force microscope (Asylum Research, Cypher S). All the electrical measurement was conducted in the dark with a semiconductor characterization system (Keithley 4200-SCS) that was connected to a probe station. For the dynamic switching performance measurement, a function generator (Tektronix AFG 3102) and a digital oscilloscope (Tektronix DPO 2024) were employed to generate the Vin and record the Vout, respectively. Both the function generator and the digital oscilloscope had common ground with the semiconductor characterization system, which provided the Vdd. All the characterizations were performed in ambient condition.
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7

Microscopic Particle Manipulation with SFIT

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A microscope (D-35578 Wetzlar, Leica, Germany) was coupled with a high speed charge-coupled-device (CCD) camera (OptiMOS, QImaging, Canada) to record the behavior of particles. MBs and polystyrene beads were injected into a microchannel by a micro-syringe pump (Pump 33; Harvard Apparatus, South Natick, MA). A dual-channel arbitrary signal generator (AFG3102, Tektronix, USA) was used to generate sinusoidal signals. The RF signals were then amplified by power amplifier (Minicircuits ZHL-1–2W, Brooklyn, NY, USA) and eventually connected to SFITs. The input power applied to the single SFITs ranged from 20 mW to 220 mW, measured by a power sensor (Minicircuits PWR-4GHS, Brooklyn, NY, USA) with a cold 30 dB attenuator.
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8

Ultrasound Neuromodulation Chip for In Vitro Stimulation

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An ultrasound neuro-modulation chip with 28 MHz resonant frequency compatible with the patch-clamp systems due to the small size and transparent character was used for stimulation of slices in vitro (Figure S5)39 -41 (link). The chip consisted of interdigital transducers (IDTs) and a recording chamber (a polydimethylsiloxane ring-shaped chamber). The finger-electrodes of IDTs were deposited using micro-electromechanical systems (MEMS) techniques on a piezoelectric 128o Y-rotated, X-propagating lithium niobate (LN, LiNbO3) sub-strate (1 mm thick, transparent) with an aluminium layer of 200 nm 42 . Pulsed ultrasound waveforms were generated by an arbitrary waveform generator (AFG 3102, Tektronix, Beaverton, Oregon) and amplified by a power amplifier (ZHL-1-2W+, Mini-Circuits, Brooklyn, NY, USA). The displacement of the piezoelectric substrate perpendicular to the surface plane was 20 pm measured by a Laser Doppler Velocimetry (UHF-120 Ultra High-Frequency Vibrometer, Polytec, Germany) and the acoustic pressure equal to 0.13 MPa (the spatial-peak pulse-average intensity (ISPPA) was evaluated to be approximately 465 mW/cm2. The spatial peak time average intensity (ISPTA) was equal to 233 mW/cm2 and was calculated by multiplying duty cycle to the ISPPA43 .
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9

Polypyrrole Nanomaterial Synthesis via APPJ

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Polypyrrole (PPy) nanomaterials were synthesized from a pyrrole monomer solution using the proposed electrode-embedded APPJ device. The amount of liquid pyrrole per treatment was 25 mL, and an Ar flow rate of 500 standard cubic centimeters per minute was used. A bipolar pulse with an amplitude of 7 kV and frequency of 5 kHz was applied to the APPJ device using a high-voltage power amplifier (20/20C-HS, Trek, Inc., Lockport, NY, USA) and function generator (AFG-3102, Tektronix Inc., Beaverton, OR, USA). The pulse duration of positive and negative polarity in bipolar pulse was equal to 100 μs and the polymerization duration time was up to 6 h.
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10

Transistor Characterization via Semiconductor Analysis

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All measurements were performed under ambient conditions. The transistor properties were measured using a semiconductor parameter analyzer (Keithley 4200-SCS). Dynamic measurements to evaluate the cutoff frequency were conducted using the same setup as that reported previously (32 ). ac signals with a peak-to-peak voltage of 1 V [vg(t)] were generated using a function generator (Tektronix AFG3102), and dc gate (VG) and drain (VD) voltages of −10 V were generated using the same semiconductor parameter analyzer. The synthesis voltage [vg(t) + VG] was applied to the gate electrode using a bias tee (ZFBT-4R2GW+, Mini Circuits). The output gate and drain currents were measured using an oscilloscope (Tektronix MDO3014) with current probes (Tektronix CT-6). Measurement of the rectifying characteristics was performed using another function generator (Tabor Electronics WS8102) to apply an input voltage, and an oscilloscope (Teledyne LeCroy HDO4054) was used to read the input and output current signals.
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