Iscript and miscript cdna synthesis kits

Staphylococcus enterotoxin B (SEB) was purchased from Toxin Technologies (Sarasota, FL, USA). Delta‐9‐Tetrahydrocannabinol (THC) was procured from (NIH National Institute on Drug Abuse, National Institutes of Health, Bethesda, MD, USA). The following reagents (culture medium reagents: RPMI 1640, L‐Glutamine, Penicillin‐Streptomycin, HEPES, FBS, and PBS) were purchased from Invitrogen Life Technologies (Carlsbad, CA). We purchased the following antibodies and Fix/Perm buffer from Biolegend (San Diego, CA, USA). Fc Block reagent was purchased from BD Pharmingen (Carlsbad, CA). True‐Nuclear™ Transcription Factor Buffer Set was purchased from BioLegend. RNeasy and miRNAeasy Mini kits, miScript primer assays kit, and miScript SYBR Green PCR kit were purchased from QIAGEN (QIAGEN, Valencia, CA). iScript and miScript cDNA synthesis kits were purchased from Bio‐Rad (Madison, WI) and Epicentre’s PCR premix F and Platinum Taq DNA Polymerase kits were purchased from Invitrogen Life Technologies (Carlsbad, CA). Ficoll‐Histopaque®‐1077 (Sigma‐Aldrich) ELISA kits for (ELISA MAXTM Standard SET Mouse) were purchased from Biolegend. Immunofluorescence antibodies were purchased from Abcam (Cambridge, MA) PBS twice.