Sp5 multi line inverted confocal microscope

Manufactured by Leica

The SP5 multi-line inverted confocal microscope is a high-performance imaging system designed for advanced microscopy applications. It features a modular design and supports multiple laser lines, enabling researchers to capture detailed images and perform complex analyses of samples.

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Lab products found in correlation

Lsm 700 confocal system, by Zeiss (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Anti p smad2 3, by Cell Signaling Technology (1 mentions) Tcs sp8, by Leica (1 mentions) Dmi8 inverted confocal microscope navigator module, by Leica (1 mentions)

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SP5 confocal microscope (1772 citations) Confocal microscope (1146 citations) Inverted microscope (717 citations) SP5 microscope (176 citations) SP5 inverted confocal microscope (153 citations) Inverted confocal microscope (20 citations) Confocal SP5 (6 citations) Inverted SP5 microscope (3 citations) Inverted Confocal SP5 (2 citations) SP5 Inverted (2 citations)

2 protocols using sp5 multi line inverted confocal microscope

Immunofluorescence Analysis of LSK and CXCL12 Cells

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CXCL12-expressing niche cells and LSK cells sorted from WT of Glg1^−/− transplanted mice were cytospun onto glass slides, immediately fixed with 2% paraformaldehyde and permeabilized with cold methanol. After blocking, cells were stained with anti-pSmad2/3 (Clone: D27-F4; Cell Signalling Technology) or anti-ESL-1rabbit serum, and secondary antibody (goat anti-rabbit Alexa Fluor 555 or Alexa Fluor 488, respectively; Invitrogen). Stained cells were counterstained with 4,6-diamidino-2-phenylindole (DAPI) to reveal nuclei. LSK cells images were collected using a Leica SP5 multi-line inverted confocal microscope. CXCL12-expressing cells images were acquired with a Zeiss LSM 700 confocal system (Carl Zeiss MicroImaging).

Leiva M., Quintana J.A., Ligos J.M, & Hidalgo A. (2016). Haematopoietic ESL-1 enables stem cell proliferation in the bone marrow by limiting TGFβ availability. Nature Communications, 7, 10222.

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Multimodal Microscopy for Cardiac Imaging

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To detect superficial signal, tissues were dehydrated in gradual concentrations of glycerol (20%, 40%, 60% 80%). For thick samples or deep signal, tissues were cleared with cubic 1 solution as described in the literature (Susaki et al., 2014 (link)).
Depending on the specific samples, experiments were performed in the following microscopes:

Leica SP5 multiline inverted confocal microscope for E16.5 and P0 fluorescent hearts in whole-mount.

Leica SP8 inverted confocal equipped with white light laser for all embryonic fluorescent sections.

Leica TCS SP8 coupled to a DMi8 inverted confocal microscope Navigator module equipped with white light laser. We used this microscope to acquire several embryonic hearts from a same experiment at the same time and ensure comparable settings for further quantifications (E15.5 RA-induced hearts, E16.5 Raldh2^f/f; Wt1^Cre mutant hearts and E17.5 isl1^Cre; R26R^tdTomato hearts).

Zeiss LSM 780 multiphoton upright microscope. A MaiTai laser line at 1000 nm was used for two-channel two-photon imaging of td-Tomato and GFP fluorescence of E15.5 and E16.5 hearts in whole mount.

Maximum intensity Z-projections of whole hearts were acquired using both the tiling and z-stack functions, generating a single composite image.

Lioux G., Liu X., Temiño S., Oxendine M., Ayala E., Ortega S., Kelly R.G., Oliver G, & Torres M. (2020). A Second Heart Field-derived vasculogenic niche contributes to cardiac lymphatics. Developmental cell, 52(3), 350-363.e6.

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