Each hippocampus was dissected from brain tissue on ice and then homogenized with iced PBS to a 10% (w/v) homogenate. The activities of IFN-γ, IL-10, IL-4, IL-6 and TNF-α were determined using the multi-analyte ELISArray Kit (SABiosciences, Frederick, MD, USA) according to the manufacturer’s instructions. Protein concentration was determined by the BCA (bicinchonininc acid) method.
Elisarray kit
Manufactured by Qiagen
Sourced in Germany, United States
The ELISArray kit is a laboratory equipment product designed for enzyme-linked immunosorbent assay (ELISA) applications. It provides a comprehensive solution for the detection and quantification of various analytes in a sample. The kit includes all the necessary components to perform ELISA tests, enabling researchers to efficiently analyze their samples.
Automatically generated - may contain errors
Lab products found in correlation
Duoset elisa development kit, by R&D Systems (1 mentions)
Human tnf α elisa kit, by Thermo Fisher Scientific (1 mentions)
Human ccl20 mip 3 alpha quantikine elisa kit, by R&D Systems (1 mentions)
Duoset elisa, by R&D Systems (1 mentions)
Human il 6 cytoset, by Thermo Fisher Scientific (1 mentions)
Microplate reader, by Molecular Devices (1 mentions)
9 protocols using elisarray kit
1
Hippocampal Cytokine Profiling via ELISA
2
Cytokine Profile After Irradiation
3
Quantifying Immune Factors in Ascites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cytokines and chemokines were quantified in AF from patients with SBP and in the absence of SBP. TNF was determined by using human TNF-α ELISA Kit (Invitrogen), CCL20 was determined by using human CCL20/MIP-3 alpha Quantikine ELISA Kit (R & D Systems, Minneapolis, MN), IL-17, IFN-γ, CXCL10, and CCL5 (RANTES) were determined by using a customized human multi-analyte ELISArray kit (Qiagen, Hilden, Germany), all according to the manufacturer’s manual.
4
Cytokine Profiling of Leukocyte-MSC Coculture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The leukocytes were cocultured with MSCs at different passages for 72 h at a ratio of 1:10 (MSCs:leukocytes). The cytokine secretion profile of leukocytes was analyzed using a multianalyte rat cytokine ELISArray kit (Qiagen; MER 336161) following instructions from the manufacturer. We analyzed the levels of 12 different cytokines including interleukin (IL)-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12, IL-13, interferon (IFN)-δ, tumor necrosis factor (TNF)-α, granulocyte-macrophage colony-stimulating factor (GM-CSF), and RANTES. The plate was read at 450 and 570 nm using a Cytation5 analyzer (BioTek Inc.) in plate reader mode.
5
Inflammatory Cytokine Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cell supernatant and mouse skin tissues were collected and centrifuged at 1000 g for 10 min, followed by 10000 g for 30 min, and the supernatant was taken to a clean EP tube. The levels of cytokines in the samples were then analyzed using an inflammatory cytokine multi analyte ELISArray Kit (Qiagen, Germany) according to the manufacturer’s instructions. Absorbance (450 nm) was measured using a multifunctional microplate reader.
6
Cytokine Release in Aβ42 Exposure
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Levels of IL-1β, TNF-α and IL-10 released from BV-2 cells after uptake of Aβ42 species of various structures were examined by enzyme-linked immunosorbent assay (ELISA). A 96-well tailor-made mouse multi-analyte ELISArray kit (Qiagen, SA Biosciences, Hilden, UK) was employed to determine the concentrations of TNF-α, IL-1β, and IL-10 in the collected culture media according to the manufacturer’s instruction.
7
Multiplex cytokine and chemokine analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Conditioned media from cultured cells were centrifuged at 15,000g for 5 min and supernatants were frozen at − 80 °C. The concentrations of G-CSF, growth-regulated oncogene alpha (GROα), monocyte chemoattractant protein-1 (MCP-1), and interleukin 6 (IL-6) were evaluated using specific ELISA assays for G-CSF, GROα, MCP-1 (Duo Set ELISA, R&D Systems, Minneapolis, MN, USA) and IL-6 (Human IL-6 CytoSet, Invitrogen), following the manufacturers’ protocols. The secreted levels of IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-13, IL-17A, IFNγ, TNFα, G-CSF, and TGF-β1 and of IL-8, MCP-1, RANTES, MIP-1α, MIP-1β, IP-10, I-TAC, MIG, Eotaxin, TARC, MDC, and GROα were qualitatively assessed with the human Th1/Th2/Th17 cytokines and the human common chemokines multianalyte ELISArray kits, respectively (Qiagen, Hilden, Germany). The quantification was performed in duplicate for each analyzed culture medium.
8
Simultaneous Cytokine Quantification by ELISA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To measure the secretion of cytokines in the culture supernatant of the in vitro stimulated cells, we used multianalyte inflammatory cytokine ELISArray kits (Qiagen, Germantown, MD, USA). The levels of IL-2, IL-8, IL-10, IL-12 and IFN-γ, pro- and anti-inflammatory cytokines were simultaneously analyzed according to the manufacturer’s instructions. Briefly, 50 µL of the cell culture supernatant was added to the appropriate wells of the ELISArray plate, which included all specific cytokine/chemokine capture antibodies. After 2 h of incubation at room temperature (RT), the plate was washed, and 100 µL of detection antibody was added to the appropriate wells and incubated for 1 h at RT. Subsequently, 100 μL of diluted avidin-horseradish peroxidase (HRP) was added to all wells and incubated at RT for 30 h. After washing, 100 μL of the development solution was added to each well and incubated for 15 minutes at RT in the dark. Finally, 100 μL of stop solution was added to each well, and the absorbance of the obtained products at 450 nm was measured. The cytokine/chemokine levels are expressed in pg/mL.
9
Quantifying Inflammatory Cytokine Profiles
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Supernatant from macrophage cultures were harvested and measured for IL-1β, IL-6, IL-12, TNFα, CCL2, CXCL9, CXCL10, CXCL11, IL-10, CCL17, and CCL22 expression using custom multi-analyte ELISArray kits (Qiagen), according to the manufacturer’s instructions. Samples were diluted to stay within the dynamic range of the assay and the dilution factors were accounted for when calculating the corrected absorbance values. Absorbance was measured using a microplate reader (Molecular Devices).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!