Total RNA was isolated from LRs of tea seedlings using plant RNA extraction kit with DNase (TIANDZ, Inc., Beijing, China) according to the manufacturer’s protocol. The quantity and quality of the RNA samples were determined by using NanoDrop2000 Spectrophotometer (Thermo Fisher Scientific, USA), 1.2% agarose gel electrophoresis, and Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA). Quality RNA samples were used for library construction and sequencing using PE150 using Illumina HiSeq platform. A total of eighteen libraries were constructed and sequenced. The experiment was replicated three times. All sequenced data have been deposited into NCBI’s Sequence Read Archive under the GenBank accession number SRA number SUB6669244.
Plant rna extraction kit with dnase
Manufactured by Tiandz
Sourced in China
The Plant RNA Extraction Kit with DNase is a laboratory tool designed for the purification of high-quality total RNA from plant tissues. The kit includes reagents and protocols for efficient RNA extraction while removing contaminating DNA.
Automatically generated - may contain errors
5 protocols using plant rna extraction kit with dnase
1
Transcriptome Profiling of Tea Seedlings
2
RNA Extraction and Sequencing Protocol
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The total RNA of LH, CK, HN was isolated with the plant RNA extraction kit with DNase (TIANDZ, Inc., Beijing, China) according to the manufacturer’s protocol. RNA was purified and concentrated using NanoDrop2000 Spectrophotometer (Thermo Fisher Scientific, USA), 1.2% agarose gel electrophoresis, and Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA). High-quality RNA samples are used for library construction, and then the total RNA-seq library is constructed and sequenced using the IlluminaHiSeq platform. All sequencing data were deposited in the National Center for Biotechnology Information (NCBI) Sequence Read Archive (accession number PRJNA595712).
3
Lateral Root Analysis of Tea Plants
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The lateral roots of each tea plant were cut down and laid at on a at plate. The WinRHIZO root analysis system (Regent Instruments, Inc., Canada) was used to scan the lateral roots and record the total number and length of the lateral roots of each tea plant. Three biological replicates were used per each sample and each measurement was replicated three times.
RNA isolation, library construction and RNA sequencing Total RNA was isolated from LRs of tea seedlings using plant RNA extraction kit with DNase (TIANDZ, Inc., Beijing, China) according to the manufacturer's protocol. The quantity and quality of the RNA samples were determined by using NanoDrop2000 Spectrophotometer (Thermo Fisher Scienti c, USA), 1.2 % agarose gel electrophoresis, and Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA). Quality RNA samples were used for library construction and sequencing using PE150 using Illumina HiSeq platform. A total of eighteen libraries were constructed and sequenced. The experiment was replicated three times. All sequenced data have been deposited into NCBI's Sequence Read Archive under the GenBank accession number SRA number SUB6669244.
RNA isolation, library construction and RNA sequencing Total RNA was isolated from LRs of tea seedlings using plant RNA extraction kit with DNase (TIANDZ, Inc., Beijing, China) according to the manufacturer's protocol. The quantity and quality of the RNA samples were determined by using NanoDrop2000 Spectrophotometer (Thermo Fisher Scienti c, USA), 1.2 % agarose gel electrophoresis, and Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA). Quality RNA samples were used for library construction and sequencing using PE150 using Illumina HiSeq platform. A total of eighteen libraries were constructed and sequenced. The experiment was replicated three times. All sequenced data have been deposited into NCBI's Sequence Read Archive under the GenBank accession number SRA number SUB6669244.
4
Transcriptomic Analysis of Tea Plant Lateral Roots
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The lateral roots of each tea plant were cut down and laid at on a at plate. The WinRHIZO root analysis system (Regent Instruments, Inc., Canada) was used to scan the lateral roots and record the total number and length of the lateral roots of each tea plant. Three biological replicates were performed for each sample.
RNA isolation, library construction and RNA sequencing Total RNA was isolated from LRs of tea seedlings using plant RNA extraction kit with DNase (TIANDZ, Inc., Beijing, China) according to the manufacturer's protocol. The quantity and quality of the RNA samples were determined by using NanoDrop2000 Spectrophotometer (Thermo Fisher Scienti c, USA), 1.2 % agarose gel electrophoresis, and Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA). Then, RNA samples were sent to Genepioneer Biotech Corporation (Nanjing, China) for library construction and sequencing. Eighteen libraries were constructed and sequenced with PE150 using Illumina HiSeq platform. Each sample was sequenced with three biological replications. All sequenced data have been deposited to the NCBI Sequence Read Archive under the GenBank accession number SRA number (uploading).
RNA isolation, library construction and RNA sequencing Total RNA was isolated from LRs of tea seedlings using plant RNA extraction kit with DNase (TIANDZ, Inc., Beijing, China) according to the manufacturer's protocol. The quantity and quality of the RNA samples were determined by using NanoDrop2000 Spectrophotometer (Thermo Fisher Scienti c, USA), 1.2 % agarose gel electrophoresis, and Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA). Then, RNA samples were sent to Genepioneer Biotech Corporation (Nanjing, China) for library construction and sequencing. Eighteen libraries were constructed and sequenced with PE150 using Illumina HiSeq platform. Each sample was sequenced with three biological replications. All sequenced data have been deposited to the NCBI Sequence Read Archive under the GenBank accession number SRA number (uploading).
5
Lateral Root Transcriptome Analysis in Tea Plants
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The lateral roots of each tea plant were cut down and laid at on a at plate. The WinRHIZO root analysis system (Regent Instruments, Inc., Canada) was used to scan the lateral roots and record the total number and length of the lateral roots of each tea plant. Three biological replicates were performed for each sample.
RNA isolation, library construction and RNA sequencing Total RNA was isolated from LRs of tea seedlings using plant RNA extraction kit with DNase (TIANDZ, Inc., Beijing, China) according to the manufacturer's protocol. The quantity and quality of the RNA samples were determined by using NanoDrop2000 Spectrophotometer (Thermo Fisher Scienti c, USA), 1.2 % agarose gel electrophoresis, and Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA). Then, RNA samples were sent to Genepioneer Biotech Corporation (Nanjing, China) for library construction and sequencing. Eighteen libraries were constructed and sequenced with PE150 using Illumina HiSeq platform. Each sample was sequenced with three biological replications. All sequenced data have been deposited to the NCBI Sequence Read Archive under the GenBank accession number SRA number SUB6669244.
RNA isolation, library construction and RNA sequencing Total RNA was isolated from LRs of tea seedlings using plant RNA extraction kit with DNase (TIANDZ, Inc., Beijing, China) according to the manufacturer's protocol. The quantity and quality of the RNA samples were determined by using NanoDrop2000 Spectrophotometer (Thermo Fisher Scienti c, USA), 1.2 % agarose gel electrophoresis, and Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA). Then, RNA samples were sent to Genepioneer Biotech Corporation (Nanjing, China) for library construction and sequencing. Eighteen libraries were constructed and sequenced with PE150 using Illumina HiSeq platform. Each sample was sequenced with three biological replications. All sequenced data have been deposited to the NCBI Sequence Read Archive under the GenBank accession number SRA number SUB6669244.
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