Cells were incubated with 1,000 vge/cell of HPV11 PsVs for indicated times and approximately 30 μg of cell lysate protein were subjected to western blotting protocols as described previously [28 (link)], using the following antibodies: polyclonal rabbit anti-LC3B (Cat#: L7543) and monoclonal mouse anti-actin (Sigma, Cat#: A9357), monoclonal rabbit anti-p62 (MBL Life science, Cat#: PM045), monoclonal rabbit anti-phospho-mTOR (Ser2448, Cat#: 2971), monoclonal rabbit anti-mTOR (Cat#: 2983), anti-phospho-70S6 kinase (Cat#: 9234), monoclonal rabbit anti-70S6 kinase (Cat#: 2708), monoclonal rabbit anti-phospho-4E-BP1 (Thr37/46, Cat#: 2855), monoclonal rabbit anti-4E-BP1 (Cat#: 9452), monoclonal rabbit anti-Erk (Cat#: 4695), monoclonal rabbit anti-phospho-Erk (Thr202/Tyr204, Cat#: 4370), monoclonal rabbit anti-Akt (Cat#: 4691), monoclonal rabbit anti-phospho-Akt (Ser473, Cat#: 4060), monoclonal rabbit anti-ULK1 (Cat#: 8054), monoclonal rabbit anti-phospho-ULK1 (Ser555, Cat#: 5869), monoclonal rabbit anti-phospho-ULK1 (Ser757, Cat#: 14202), monoclonal rabbit anti-AMPK (Cat#: 2603) and monoclonal rabbit anti-phospho-AMPK (Ser172, Cat#: 2531) (Cell Signaling Technology).
Han R., Hua C., Sun S., Zhang B., Song Y., van der Veen S, & Cheng H. (2020). Autophagy is induced in human keratinocytes during human papillomavirus 11 pseudovirion entry. Aging (Albany NY), 12(22), 23017-23028.
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