Buffer A was prepared following a method previously reported in a study by I.ssewanyana et al. Briefly, 1L of phosphate buffer solution was measured and put in a conical flask after which 500μl of tween (Sigma-Aldrich; USA) was dispensed into the PBS to form PBS-tween solution. 5g of PVP powder (Sigma-Aldrich; USA) was weighed using a weighing balance in aluminum foil and added to the above solution after which equal amount of PVA powder was weighed and added to the mixture; proper agitation and mixing was done using a vortexer. 5mls of BSA (Thermofisher Scientific;USA) was pipetted and added to the solution; finally, 0.2g of Sodium azide (Sigma-Aldrich; USA) was weighed under a biosafety hood in an aluminum foil, added to the solution, properly vortexed and mixed. The final solution was labelled with day of preparation and names of those who prepared.
Pvp powder
Manufactured by Merck Group
Sourced in United States, Canada
PVP powder is a synthetic polymer used as a stabilizing and suspending agent in various pharmaceutical and industrial applications. It is soluble in water and other polar solvents, and is commonly used to enhance the solubility and stability of active ingredients in drug formulations.
Automatically generated - may contain errors
Lab products found in correlation
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
Sodium azide, by Merck Group (1 mentions)
Pva powder, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Fe powder, by Merck Group (1 mentions)
Hexane, by Merck Group (1 mentions)
H2so4, by Merck Group (1 mentions)
Deionized water, by Merck Group (1 mentions)
1 3 5 benzenetricarbonyltrichloride tmc, by Merck Group (1 mentions)
Anhydrous dimethylformamide dmf, by Merck Group (1 mentions)
4 protocols using pvp powder
1
Preparation of Phosphate Buffered Saline-Tween Polymer Solution
2
Synthesis of Nano-FeF2 Powder via FeSiF6 Precursor
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In a typical synthesis process, excess amount of Fe powder (2.0 g, Sigma‐Aldrich, 99%) was reacted with 10 mL of H2SiF6 aqueous solution (25wt%, Sigma‐Aldrich) for 24 h. The mixture was then centrifuged to harvest the solids. The as‐received solids were then diluted to 20 mL with distilled water to get a FeSiF6 aqueous solution. 3 g PVP powder (Sigma‐Aldrich, average Mw ≈6500) was dissolved in 20 mL ethanol (Sigma‐Aldrich, 99.8%) and stirred for 2 h. Then the as‐prepared FeSiF6 and PVP alcohol solution was mixed and stirred for 2 h to obtain precipitates. Afterward, the precipitates were dried in oven under vacuum at 60°C overnight. To completely remove the solvent, the obtained powder was grinded and further dried at 150°C in vacuum. Finally, the powder was annealed at 260°C for 4 h followed by annealing at 550°C for 3 h under an argon flow. To obtain nano‐FeF2 powder without PDC, the FeSiF6 aqueous solution was dried and annealed at 260°C for 4 h under an argon flow, in which the following chemical reaction takes place: FeSiF6 → FeF2 + SiF4 (gas).
3
Fabrication of MWCNT-Embedded Polyamide Membranes
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PSF
(average molecular
weight Mn ≈ 22,000, MO, Sigma-Aldrich) was used for the membrane
support layer. PVP powder (average molecular weight: 360,000, Sigma-Aldrich)
was used as a pore former. Anhydrous dimethyl formamide (DMF) (density:
0.944 g/mL, 99.8% purity, Sigma-Aldrich) was used as a solvent. Deionized
water (MilliPore) was used as a nonsolvent. For the PA rejection layer, m-phenylenediamine (MPD) flakes (molecular weight: 108.14
g/mol, 99% purity, Sigma-Aldrich) and 1,3,5-benzenetricarbonyltrichloride
(TMC) (molecular weight: 265.48 g/mol, 98% purity, Sigma-Aldrich)
were used as reacting monomers. Deionized water (MilliPore) was used
as a solvent for MPD. Hexane (density: 0.672, purity >98.5%, Sigma-Aldrich)
was used as a solvent for TMC. Elicarb MWCNTs (diameter: 10–12
nm, length: tens of micrometers, and density: 1.7–1.9 g/cm3, manufactured by Thomas Swan, England) were used as nanofillers.
H2SO4 (98% purity, Sigma-Aldrich) and HNO3 (purity >69%, Sigma-Aldrich) were used in the oxidation
functionalization
of MWCNTs. Sodium chloride (purity ≥99.5%, FLUKA) was used
for evaluating salt rejection.
(average molecular
weight Mn ≈ 22,000, MO, Sigma-Aldrich) was used for the membrane
support layer. PVP powder (average molecular weight: 360,000, Sigma-Aldrich)
was used as a pore former. Anhydrous dimethyl formamide (DMF) (density:
0.944 g/mL, 99.8% purity, Sigma-Aldrich) was used as a solvent. Deionized
water (MilliPore) was used as a nonsolvent. For the PA rejection layer, m-phenylenediamine (MPD) flakes (molecular weight: 108.14
g/mol, 99% purity, Sigma-Aldrich) and 1,3,5-benzenetricarbonyltrichloride
(TMC) (molecular weight: 265.48 g/mol, 98% purity, Sigma-Aldrich)
were used as reacting monomers. Deionized water (MilliPore) was used
as a solvent for MPD. Hexane (density: 0.672, purity >98.5%, Sigma-Aldrich)
was used as a solvent for TMC. Elicarb MWCNTs (diameter: 10–12
nm, length: tens of micrometers, and density: 1.7–1.9 g/cm3, manufactured by Thomas Swan, England) were used as nanofillers.
H2SO4 (98% purity, Sigma-Aldrich) and HNO3 (purity >69%, Sigma-Aldrich) were used in the oxidation
functionalization
of MWCNTs. Sodium chloride (purity ≥99.5%, FLUKA) was used
for evaluating salt rejection.
4
Sperm Preparation Using 10% PVP
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10% polyvinylpyrrolidone (PVP) was prepared by dissolving PVP powder (1 g) (Sigma Aldrich, Canada) in PureSperm wash (10 mL) (Nidacon, Canada). Media (70 μL) used for each test consisted of a mixture with PureSperm wash (30 μL) and 10% PVP (40 μL).
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