Trance rankl tnfsf11 quantikine elisa kit

Whole blood was isolated from the submandibular vein and subsequently centrifuged at 2500 × g for 20 minutes at 4°C to separate the serum. IGF-1, Osteoprotegerin, and RANKL concentrations within the sera were quantified using the Mouse IGF-1 Quantikine ELISA kit, Osteoprotegerin/TNRSF11B Quantikine ELISA kit, and TRANCE/RANKL/TNFSF11 Quantikine ELISA kit (all R&D Systems, Minneapolis, MN), respectively. GH levels in the sera were quantified using the Milliplex pituitary magnetic bead assay (EMD Millipore, Billerica, MA). IGF-1 was measured at multiple ages (2, 12, and 27 months of age), while OPG and RANKL were measured at 27 months of age. GH was measured at 7 and 27 months of age. All analytes were measured in replicate following the manufacturers’ recommendations.