Log In Sign up
The largest database of trusted experimental protocols

Brilliant green

Manufactured by Merck Group
Visit Supplier
Sourced in United States, Germany

Brilliant green is a synthetic dye used as a laboratory reagent. It is a green crystalline solid with the chemical formula C27H34N2O4S.

Automatically generated - may contain errors

Lab products found in correlation

Malachite green, by Merck Group (2 mentions) Rhodamine b, by Merck Group (2 mentions) Crystal violet, by Merck Group (2 mentions) Methanol, by Merck Group (2 mentions) Orange 2 sodium salt, by Merck Group (1 mentions) Ponceau s, by Merck Group (1 mentions) Auramine o, by Merck Group (1 mentions) Congo red, by Merck Group (1 mentions) Reactive blue 19, by Merck Group (1 mentions) Reactive green 19, by Merck Group (1 mentions) Reactive black 5, by Merck Group (1 mentions) Peasy t3 vector, by Transgene (1 mentions) Pet28a, by Takara Bio (1 mentions) Reactive red 120, by Merck Group (1 mentions) Gentian violet, by Merck Group (1 mentions) Tempol, by Enzo Life Sciences (1 mentions) Rnai oligonucleotides, by Horizon Discovery (1 mentions) Dharmafect 1 transfection reagent, by Horizon Discovery (1 mentions) Remazol brilliant blue r, by Merck Group (1 mentions) Allura red, by Merck Group (1 mentions)

Close spelling variants of this product

Brilliant green agar (5 citations) Brilliant green dye (2 citations) Brilliant Green Bile Broth media (2 citations) Brilliant-Green Phenol-Red Lactose Sucrose Agar (4 citations) Brilliant SYBR Green PCR Master Mix (2 citations)

10 protocols using brilliant green

1

Dye Analysis for Textile Conservation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rhodamine
B (CI 45170) (1),
aniline yellow (CI 11000) (2), Congo red (CI 22120) (3), orange II sodium salt (CI 15510) (4), ponceau
S (CI 27195) (5), xylidine ponceau (CI 16150) (6), auramine O (CI 41000) (7), brilliant green
(CI 42040) (8), malachite green (CI 42000) (9), and naphthol yellow S (CI 10316) (10) were obtained
from Sigma-Aldrich Inc., St. Louis, MO, USA. Methylene blue (CI 52015)
(11), basic fuchsin (CI 42510) (12), methyl
violet 2B (CI 42535) (13), and Martius yellow (CI 10315)
(14) were purchased from Fluorochem Ltd., Hadfield, UK.
Undyed, degummed, unmordanted silk (2-ply, 66 Tex, thread count 43
cm–2) and undyed, washed, unmordanted wool cloth
(3-ply, 158 Tex, thread count 36 cm–2) from the
Monitoring of Damage to Historic Tapestries project (MODHT) (FP5,
EC contract number EVK4-CT-2001-00048)26 ,27 and undyed
cotton cloth locally purchased (thread count 32 cm–2) were used as the reference cloths (microscope images of reference
cloths in Figure S1, ESI). The H2O, MeOH, and CH3CN (ACN) (LC–MS grade) were purchased
from Fisher Scientific, Waltham, MA, USA. Dye samples from Lehne’s
handbook, 1893 (Figure 2), were also analyzed. Fabric clips (Prym Love clips, 1.0 ×
2.6 cm) were bought locally, while water-sensitive paper (Pentair
Hypro) was purchased from Agratech NW Ltd., Rossendale, UK.
Sandström E., Vettorazzo C., Mackay C.L., Troalen L.G, & Hulme A.N. (2023). Development and Application of Desorption Electrospray Ionization Mass Spectrometry for Historical Dye Analysis. Analytical Chemistry, 95(11), 4846-4854.
+ Open protocol
+ Expand
2

Cultivation and Expression of Saccharomonospora viridis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Saccharomonospora viridis DSM 43017 was obtained from the China General Microbiological Culture Collection Center, Beijing (reference number CGMCC 4.1324). The strain was cultivated in a shaker flask at 45°C in STS medium (1.0% (w/v) soy peptone, 1.0% (w/v) glucose, 0.2% (w/v) yeast extract, 0.2% (w/v) NaCl, and 0.2% casein enzymatic hydrolysate, all from Biodee, Beijing, China), adjusted pH to 8.0 with NaOH prior to autoclaving.
Escherichia coli DH5α and E. coli BL21 (Tiangen, Beijing, China) (were cultivated in Luria Bertani (LB) medium at 37°C for gene cloning, sequencing, and expression. The pEASY-T3 vector (TransGen, Beijing, China) was used for plasmid gene cloning and sequencing. The plasmid pET-28a (+) (Takara Bio, Otsu, Japan) was used as an expression vector. Manganese peroxidase (MnP), azure B, brilliant green, reactive blue 19, reactive green 19, reactive yellow 2, reactive black 5, reactive red 120, malachite green and crystal violet were purchased from Sigma (St. Louis, MO, USA). All other reagents used were of analytical grade unless otherwise stated.
Yu W., Liu W., Huang H., Zheng F., Wang X., Wu Y., Li K., Xie X, & Jin Y. (2014). Application of a Novel Alkali-Tolerant Thermostable DyP-Type Peroxidase from Saccharomonospora viridis DSM 43017 in Biobleaching of Eucalyptus Kraft Pulp. PLoS ONE, 9(10), e110319.
+ Open protocol
+ Expand
3

Native PAGE and Zymogram Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
In addition to SDS PAGE,
native PAGE followed by a zymogram was run
to demonstrate the loss of activity in the propeptide-containing sample
(Figure 8). The compositions for native PAGE were as follows.
Resolving gel: 12% acrylamide-bis-acrylamide; 0.4 M Tris (pH 8.8),
0.1% APS, 0.04% TEMED. Stacking gel: 5.1% acrylamide-bis-acrylamide;
0.25 M Tris (pH 6.8), 0.1% APS, 0.1% TEMED. Loading dye: 0.06 M Tris
(pH 6.8), 0.01% bromophenol blue, 10% glycerol. Running buffer: 3
g/L Tris base, 14.4 g/L glycine. Gel (B) is stained with brilliant
blue. Zymogram: After electrophoresis, the native PAGE was placed
on an agarose/brilliant blue plate (4% Litex HSB agarose protein grade,
50 mM HEPES (pH 7), 0.625% polyvinyl alcohol, 1.9% (v/v) olive oil, and 0.05% brilliant green
(Sigma)).
Moroz O.V., Blagova E., Reiser V., Saikia R., Dalal S., Jørgensen C.I., Bhatia V.K., Baunsgaard L., Andersen B., Svendsen A, & Wilson K.S. (2019). Novel Inhibitory Function of the Rhizomucor miehei Lipase Propeptide and Three-Dimensional Structures of Its Complexes with the Enzyme. ACS Omega, 4(6), 9964-9975.
+ Open protocol
+ Expand
4

Modulating Cell Signaling with Antioxidants

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gentian Violet and Brilliant Green were purchased from Sigma-Aldrich. Tempol was from Enzo Life Sciences. Previously described RNAi oligonucleotides (RNAi, [35] (link)) and Dharmafect 1 transfection reagent were from Dharmacon.
Mukawera E., Chartier S., Williams V., Pagano P.J., Lapointe R, & Grandvaux N. (2015). Redox-modulating agents target NOX2-dependent IKKε oncogenic kinase expression and proliferation in human breast cancer cell lines. Redox Biology, 6, 9-18.
+ Open protocol
+ Expand
5

Cultivating Aerobic Granules for Dye Removal

Check if the same lab product or an alternative is used in the 5 most similar protocols
To cultivate aerobic granules, synthetic wastewater with the following composition was used: COD (sodium acetate) 1000 mg lL -1 , NH4Cl 115 mg Ll -1 , K2HPO4 45 mg Ll -1 , CaCl2•2H2O 30 mg Ll -1 , MgSO4•7H2O 25 mg Ll -1 , FeSO4•7H2O 20 mg Ll -1 , and trace elements same as those used by Chen et al. (2015) . After aerobic granular sludge reached steady state, reactive azo dye, i.e., Acid Red 14 (Chromotrope FB, Sigma-Aldrich, 50% dye content), triphenylmethane dye, i.e., Brilliant Green (Sigma-Aldrich, 90% dye content), and anthraquinone dye, i.e., Reactive Blue 19 (Remazol Brilliant Blue R, Sigma-Aldrich) was added, respectively, to the above-mentioned synthetic wastewater to simulate wastewater containing different classes of dye . The dye concentration was increased step-wise from 5 to 85 mg /L -1 during the long-term operation period to investigate dye decolourisation and granule stability.
Liu Y.Q., Maulidiany N., Zeng P, & Heo S. (2021). Decolourization of azo, anthraquinone and triphenylmethane dyes using aerobic granules: Acclimatization and long-term stability. Chemosphere, 263.
+ Open protocol
+ Expand
6

Peptide Bioink Preparation and Handling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pure peptides were dissolved in HBSS at either 30 or 40 mg/mL. Sonication was used to dissolve all the peptide, while centrifugation was used to remove any bubbles. For the 3 wt% K2, 4 wt% K2, and 4 wt% E2 peptide bioinks, Tartrazine, Allura red, and Brilliant green (Sigma Aldrich, St. Louis, MO) were dissolved in HBSS at 0.1, 0.1 and 0.01 mg/mL, respectively, prior to the addition of peptide. After being fully dissolved, the peptide bioinks were drawn into a 3mL syringe, centrifuged to remove any bubbles, and then loaded into an Allevi plastic syringe (Allevi by 3D Systems, Philadelphia, PA) via a female-to-female syringe coupler. All procedures were done under sterile conditions.
Skinner C.M, & Wiles A.J. (1997). Ordinary representations and modular forms. Proceedings of the National Academy of Sciences of the United States of America, 94(20), 10520-10527.
+ Open protocol
+ Expand
7

Chromatogram Visualization Using Diverse Agents

Check if the same lab product or an alternative is used in the 5 most similar protocols
Processes of spot visualization were carried out using several visualization agents procured from different suppliers. The solutions of these reagents were prepared as follows:
-(i) rhodamine B (POCh), (ii) Janus blue (Michrom, UK), (iii) methyl green (Fluka, Switzerland), (iv) brilliant green (POCh), (v) crystalline violet (Sigma-Aldrich), (vi) alkaline blue (Merck), (vii) gentian violet (Fluka), and (viii) methylene violet (Michrom) were used as 0.50 mg mL -1 solutions in distilled water, -fuchsine procured from Serva (Germany) was used as 0.150 mg mL -1 solution in distilled water, -brilliant cresyl blue supplied by Michrom was used as 0.50 mg mL -1 solution in 2 % aqueous NaOH.
Taking into account the visualization manner, all obtained chromatograms have been divided into four groups. The first group was dipped in an individual visualization agent for 5 s and then left at room temperature until dry. The second group was immersed in an individual visualization agent for 5 s and then dried in a laboratory dryer at 110 °C for 1 hour. The third group was sprayed with a visualization agent and left at room temperature until dry. The fourth group was sprayed with a visualization agent and dried for one hour in a laboratory dryer at 110 °C.
Parys W., Pyka-Pająk A, & Dołowy M. (2021). A cost-effective and sensitive TLC-densitometric identification of meloxicam. Acta pharmaceutica (Zagreb, Croatia), 71(1).
+ Open protocol
+ Expand
8

Microbial Media Preparation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The employed media including Lactose broth, Brilliant green, EC broth and R2A agar media were prepared from Merck, Germany. The Asparagine broth and Acetamide broth media were prepared from Sigma-Aldrich, USA.
Karbasdehi V.N., Dobaradaran S., Nabipour I., Ostovar A., Arfaeinia H., Vazirizadeh A., Mirahmadi R., Keshtkar M., Ghasemi F.F, & Khalifei F. (2017). Indicator bacteria community in seawater and coastal sediment: the Persian Gulf as a case. Journal of Environmental Health Science and Engineering, 15, 6.
+ Open protocol
+ Expand
9

Synthesis of Zn(II) Complex for Applications

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Oleic acid (99%), octadecylamine (90%), methanol (≥99.9%), toluene (≥99.5%), brilliant green (~90.0%), rhodamine B (95%), silver nitrate (≥99.0%), acrylamide (98%), and isopropanol were purchased from Merck (Darmstadt, Germany). All reagents were used as obtained without further purification. Bis(morpholinyldithiocarbamato)Zn(II) complex was prepared using reported procedure [64 (link)].
Ajibade P.A, & Oluwalana A.E. (2021). Photocatalytic Degradation of Single and Binary Mixture of Brilliant Green and Rhodamine B Dyes by Zinc Sulfide Quantum Dots. Molecules, 26(24), 7686.
+ Open protocol
+ Expand
10

Synthesis and Characterization of Dye-Doped Polymeric Nanocomposites

Check if the same lab product or an alternative is used in the 5 most similar protocols
Aniline
monomer [C6H5NH2], ammonium persulfate
(APS) [(NH4)2S2O8], hydrochloric
acid
[HCl], zinc acetate dihydrate [ZnC4H6O4], poly(ethylene glycol) (PEG; as a surfactant), monoethanolamine
[C2H7NO], l-cysteine [C3H7NO2S], methanol [CH3OH], ethanol
[C2H5OH], acetone [C3H6O], sodium hydroxide [NaOH], potassium nitrate [KNO3],
three anionic dyes, namely, methyl orange [C14H14N3NaO3S], amido black 10B [C22H14N6Na2O9S2], eriochrome
black T [C20H12N3O7SNa],
and three cationic dyes, namely, brilliant green [C27H34N2O4S], crystal violet [C25N3H30Cl], and methylene blue [C16H18ClN3S], were purchased from Merck Pvt.,
Ltd. The structures and some features of the selected dyes are provided
in Figure S1 and Table S1, respectively.
Double-distilled water (DDW) was used in all experimental processes.
Bir R., Tanweer M.S., Singh M, & Alam M. (2022). Multifunctional Ternary NLP/ZnO@l-cysteine-grafted-PANI Bionanocomposites for the Selective Removal of Anionic and Cationic Dyes from Synthetic and Real Water Samples. ACS Omega, 7(49), 44836-44850.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!