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Facsymphony s6 sorter

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The BD FACSymphony S6 is a high-performance cell sorter designed for advanced flow cytometry applications. It features six lasers and 18 fluorescence detectors, providing users with exceptional flexibility and resolution for complex multiparametric analysis and sorting.

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Lab products found in correlation

Facsaria 2, by BD (2 mentions) Tcl buffer, by Qiagen (2 mentions) β mercaptoethanol, by Merck Group (2 mentions)

Spelling variants of this product

FACSymphony (205 citations) FACSymphony S6 (18 citations) BD FACSymphony S6 cell sorter (9 citations)

2 protocols using facsymphony s6 sorter

1

Single-cell BCR and Bulk RNA-seq from GC B cells

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Cell sorting for single-cell BCR sequencing and bulk RNA-seq was performed on a BD FACSAria II. Lysis buffer was made fresh prior to each sort by supplementing TCL buffer (Qiagen) with 1% β−mercaptoethanol (Sigma-Aldrich). For single-cell BCR sequencing, single B cells were sorted into 96-well plates containing 5 μL lysis buffer. For sorting of GC populations for bulk RNA-seq, up to 400 cells, from four independent experiments, were sorted into 25 μL of lysis buffer. For single cell RNA-seq, LZ B cells, from two independent experiments, were sorted into 96-well plates containing 5 μL of lysis buffer using a BD FACSymphony S6 sorter. Samples were centrifuged and flash-frozen on dry ice.
Chen S.T., Oliveira T.Y., Gazumyan A., Cipolla M, & Nussenzweig M.C. (2023). B cell receptor signaling in germinal centers prolongs survival and primes B cells for selection. Immunity, 56(3), 547-561.e7.
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2

Single-cell BCR and Bulk RNA-seq from GC B cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell sorting for single-cell BCR sequencing and bulk RNA-seq was performed on a BD FACSAria II. Lysis buffer was made fresh prior to each sort by supplementing TCL buffer (Qiagen) with 1% β−mercaptoethanol (Sigma-Aldrich). For single-cell BCR sequencing, single B cells were sorted into 96-well plates containing 5 μL lysis buffer. For sorting of GC populations for bulk RNA-seq, up to 400 cells, from four independent experiments, were sorted into 25 μL of lysis buffer. For single cell RNA-seq, LZ B cells, from two independent experiments, were sorted into 96-well plates containing 5 μL of lysis buffer using a BD FACSymphony S6 sorter. Samples were centrifuged and flash-frozen on dry ice.
Otto-Bruc A.E., Fariss R.N., Van Hooser J.P, & Palczewski K. (1998). Phosphorylation of photolyzed rhodopsin is calcium-insensitive in retina permeabilized by α-toxin. Proceedings of the National Academy of Sciences of the United States of America, 95(25), 15014-15019.
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