Axyprep blood genomic dna minprep kit

Venous blood was taken from all participants and DNA isolated from EDTA-anticoagulated blood samples using the AxyPrep^TM Blood Genomic DNA Minprep Kit (Axygen, Union City, CA, USA). Primer Premier 5.0 software was used to design the primers for polymerase chain reaction (PCR) amplification and final primers were evaluated using NCBI-BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi). Primer sequences are given in Table 1.
DNA samples (TPH2: rs4570625, rs120074175, rs11178997; 5-HT2A: rs7997012, rs6311, rs6313) were genotyped using PCR TaqMan assays and read on an ABI PRISM 7900 Sequence Detection System (Applied Biosystems, Foster City, CA, USA). PCR amplicons were sequenced and purified using an ABI 3730 DNA Sequencer.

Genomic DNA was isolated from collected EDTA-anticoagulated blood samples using the AxyPrepTM Blood Genomic DNA Minprep Kit (Axygen, Union City, CA, USA). SNPs in same haplotype blocks, which have been reported as having strong epidemiological credibility for involvement with MDD (DVL3: rs1969253, rs1709642; GSK3β: rs334558, and rs6438552, rs2199503) were selected. Primers for polymerase chain reaction (PCR) amplification were designed with Primer 5.0 software, and final specific primers were checked using NCBI-BLAST. Probe sequences and descriptions for SNPs are displayed in Table 1. The ABI PRISM 7900 Sequence Detection System (Applied Biosystems, Foster City, CA, USA) and ABI 3730 DNA sequencer (Applied Biosystems) were used for genotyping and purifying.