T g ha vsmc

Manufactured by Procell

Sourced in China

The T/G HA-VSMC is a lab equipment product that serves as a cell culture system. It is designed for the cultivation and maintenance of vascular smooth muscle cells (VSMCs). The product provides a controlled environment for cell growth and experimentation.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions) Mir nc, by Sangon (1 mentions) Ham s f12k, by Procell (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Hek293t, by Procell (1 mentions) Hyclone, by Cytiva (1 mentions)

3 protocols using t g ha vsmc

Transfection of miR-21 in Human VSMCs

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Human VSMCs (T/G HA-VSMC) purchased from Procell Life Science & Technology (Wuhan, China) were cultured in Ham's F-12K (Procell Life Science & Technology) with 10% FBS, 100 μg/mL streptomycin, and 100 U/mL penicillin in an incubator containing 5% CO₂ at 37°C.
For cell transfection, cells were inoculated into poly-D-lysine-coated 6-well plates at the concentration of 2 × 10⁵. Then, the sequences of miR-21 and the negative control (miR-NC) designed by Sangon Biotech (Shanghai, China) were transfected into T/G HA-VSMC cells using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA).

Liu W., Li Y, & Zhao D. (2021). Modulation of Vascular Smooth Muscle Cell Multiplication, Apoptosis, and Inflammatory Damage by miR-21 in Coronary Heart Disease. Computational and Mathematical Methods in Medicine, 2021, 6942699.

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Culturing Human Vascular and Renal Cells

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Human vascular smooth muscle cells (T/G HA‐VSMC) and human renal epithelial cells (HEK‐293T) were obtained from Procell company. 10% fetal bovine serum (GIBCO BRL) was added to DMEM (GIBCO, USA), and all cells were incubated under 5% CO₂ at 37℃ in two humidified incubators.

Nie H., Zhao W., Wang S, & Zhou W. (2021). Based on bioinformatics analysis lncrna SNHG5 modulates the function of vascular smooth muscle cells through mir‐205‐5p/SMAD4 in abdominal aortic aneurysm. Immunity, Inflammation and Disease, 9(4), 1306-1320.

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Culturing Human Aortic VSMCs and HEK-293T Cells

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A human aortic VSMC line (T/G HA-VSMC) was purchased from Procell Life Science & Technology Co., Ltd.. Human aortic VSMCs were cultured in smooth muscle cell medium (SMCM; ScienCell Research Laboratories, Inc.) which contained basic medium, 2% fetal bovine serum, 1% smooth muscle cell growth supplement and 1% penicillin/streptomycin solution. A human embryonic kidney (HEK)-293T cell line was purchased from the Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (Beijing, China) and maintained in Dulbecco's modified Eagle's medium (Hyclone; Cytiva) containing 10% fetal calf serum (Gibco; Thermo Fisher Scientific, Inc.). Both cell lines were kept in a humidified atmosphere of 5% CO₂ at 37˚C.

Gan S., Mao J., Pan Y., Tang J, & Qiu Z. (2021). hsa-miR-15b-5p regulates the proliferation and apoptosis of human vascular smooth muscle cells by targeting the ACSS2/PTGS2 axis. Experimental and Therapeutic Medicine, 22(5), 1208.

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