Sf3b3

Manufactured by Abcam

Sourced in United States

SF3B3 is a splicing factor that plays a role in the regulation of pre-mRNA splicing. It is a component of the spliceosome, a complex of small nuclear ribonucleoproteins (snRNPs) and associated proteins that catalyzes the removal of introns from pre-mRNA.

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Lab products found in correlation

Mcf 7 cell, by American Type Culture Collection (1 mentions) Bafilomycin a1, by Merck Group (1 mentions) Chloroquine, by Merck Group (1 mentions) Beclin 1, by Abcam (1 mentions) β actin, by Proteintech (1 mentions) Sbi 0206965, by Merck Group (1 mentions) Goat anti mouse igg, by Cell Signaling Technology (1 mentions) Goat anti rabbit igg, by Cell Signaling Technology (1 mentions) Ripa lysis buffer, by Beyotime (1 mentions) E cadherin, by Proteintech (1 mentions) N cadherin, by Proteintech (1 mentions) Vimentin, by Proteintech (1 mentions) Phospho ikkα β, by Cell Signaling Technology (1 mentions) Phospho p65, by Cell Signaling Technology (1 mentions) Desmoplakin, by Proteintech (1 mentions) Cortactin, by Proteintech (1 mentions) N wasp, by Cell Signaling Technology (1 mentions) Phospho iκbα, by Cell Signaling Technology (1 mentions) Snail, by Cell Signaling Technology (1 mentions) Gapdh, by Proteintech (1 mentions)

3 protocols using sf3b3

Autophagy Modulation in MCF-7 Breast Cancer Cells

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MCF-7 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA) and used to conduct functional assays. The cells were cultured in DMEM medium supplemented with 10% FBS, 100 μg/mL streptomycin, 100 U/mL penicillin, and 0.03% l-glutamine and maintained at 37 °C with 5% CO₂ at a humidified atmosphere. SBI-0206965, 3-MA, bafilomycin A1 and chloroquine were purchased from Sigma–Aldrich [the detail information are as follows: SBI-0206965 (SML1540), 3-MA (189490), bafilomycin A1 (196000) and chloroquine (C6628)]. The primary antibodies used for Western blot are as follows: LC3 B (Abcam, ab192890), beclin-1 (Abcam, ab207612), SF3B3 (Abcam, ab96683), ULK1 (8054, CST, MA, USA), p-ULK1^Ser317 (12753, CST), p-ULK1^Ser555 (5869, CST), mATG13 (13273, CST), p-mATG13^Ser318 (PAB19948, Abnova, Taiwan), ATG101 (13492, CST), FIP200 (12436, CST), SQSTM1/P62 (5114, CST), ATG4B (13507, CST), ATG4C (5262, CST), ATG5 (12994, CST), ATG16L1 (8089, CST), SIRT3 (2627, CST), ac-MnSOD2 K68 (Abcam, ab137037), ac-MnSOD2 K122 (Abcam, ab214675), MMP-2(40994, CST), MMP-9 (13667, CST), E-cadherin (14472, CST), and β-actin (66009-1-Ig, Proteintech, IL, USA). In addition, the secondary infrared antibodies goat anti-rabbit IgG (Cell Signaling Technology #4410, 1:5000) and goat anti mouse IgG (Cell Signaling Technology #4414, 1:5000) were added.

Zhang S., Zhang J., An Y., Zeng X., Qin Z., Zhao Y., Xu H, & Liu B. (2020). Multi-omics approaches identify SF3B3 and SIRT3 as candidate autophagic regulators and druggable targets in invasive breast carcinoma. Acta Pharmaceutica Sinica. B, 11(5), 1227-1245.

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Comprehensive EMT Protein Analysis

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Cells were lysed with RIPA lysis buffer (Beyotime) and added the protease inhibitor. Next, the concentration of proteins was measured by a BCA Protein Quantification Kit (Yeasen). The standard western blotting protocol was followed. The primary antibodies included antibodies specific for EMT markers, Desmoplakin (Proteintech, # 25318-1-AP), ZEB2 (Proteintech, # 14026-1-AP), N-cadherin (Proteintech, # 22018-1-AP), E-cadherin (Proteintech, # 20874-1-AP), Vimentin (Proteintech, # 10366-1-AP), Slug (Signalway Antibody, #24463), Snail (Cell Signaling Technology, # 3879 T), GAPDH (Proteintech, #10494-1-AP), N-WASP (Cell Signaling Technology, # 4848 T), Phospho-N-WASP (Affinity Biosciences, # AF7032), Cortactin (Proteintech, # 11381-1-AP), SF3B3 (Abcam, # ab209402), IKKα (Cell Signaling Technology, #11930), IKKβ (Cell Signaling Technology, #8943), Phospho-IKKα/β (Cell Signaling Technology, #2697), Phospho-p65 (Cell Signaling Technology, #3033), IκBα (Cell Signaling Technology, #4814), Phospho-IκBα (Cell Signaling Technology, #2859), and p65 (Cell Signaling Technology, #8242) were detected. The antibody dilution ratios were determined according to the protocol. The reference gene was GAPDH.

Wang J., Huang T.J., Mei Y., Luo F.F., Xie D.H., Peng L.X., Liu B.Q., Fan M.L., Zhang J.B., Zheng S.T., Qian C.N, & Huang B.J. (2022). Novel long noncoding RNA LINC02820 augments TNF signaling pathway to remodel cytoskeleton and potentiate metastasis in esophageal squamous cell carcinoma. Cancer Gene Therapy, 30(2), 375-387.

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Western Blot Protein Analysis

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Standard protocols for Laemmli gels and western blotting were used, loading 50 μg of total protein for both single cells (D) and day 5–7 (P) cell lysates. Antibodies for western blotting from Abcam were U1A (ab55751), Sf3b1 (ab39578), Sf3b3 (ab96683), Srsf7 (ab138022), Tra2 β (ab66901), Rbm3 (ab134946), hnPNPL (ab6106) and Tubulin (ab6160). Antibodies from Santa Cruz Biotechnology were U1C (sc-101549), U2A’ (sc-393803), Srsf6 (sc-34198), Rbp1 (sc-56767) and Gapdh (sc-25778). The remaining antibodies were U170K/Snrnp70 Synaptic Systems (203 011), Srsf1 Invitrogen (32-4500), PTBP1 Invitrogen (324800), PTBP2 (kindly provided by Michele Solimena), QKI (UC Davis/NIH NeuroMab Facility clone N147/6), PTBP3 provided by Elisa Monzon-Casanova, Acta2 Dako (M0851) and Myh-11 Sigma (M7786).

Llorian M., Gooding C., Bellora N., Hallegger M., Buckroyd A., Wang X., Rajgor D., Kayikci M., Feltham J., Ule J., Eyras E, & Smith C.W. (2016). The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators. Nucleic Acids Research, 44(18), 8933-8950.

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