DPSCs cultured in α-MEM medium were analyzed for CD90 and CD45 cell surface antigens expression. Fluorescein isothiocyanate (FITC) - conjugated mouse anti-human CD90 and phycoerythrin (PE) conjugated mouse anti-human CD45 (BD Biosciences, USA) were utilized. DPSCs were detached from the plate using 0.25% trypsin with 1 mM EDTA (Gibco, Thermo Fisher Scientific, USA) and 50 μl of cell suspension (100,000 cells) was mixed with 5 μl of the corresponding antibody. After incubation for 30 min in the dark, the cells were washed and then acquired by flow cytometry (CYTOMICS FC 500 Flow Cytometer, Beckman Coulter, USA) and analyzed by Cyflogic v.1.2.1 software.
Fitc conjugated mouse anti human cd90
Manufactured by BD
Sourced in United States
FITC-conjugated mouse anti-human CD90 is a monoclonal antibody that binds to the CD90 (Thy-1) antigen on human cells. It is conjugated with the fluorescent dye FITC (fluorescein isothiocyanate) for detection purposes.
Automatically generated - may contain errors
Lab products found in correlation
Cytomics fc500 flow cytometer, by Beckman Coulter (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions)
Fitc conjugated mouse anti human cd45, by BD (1 mentions)
Fitc conjugated mouse anti human cd105, by BD (1 mentions)
Fitc conjugated mouse anti human cd34, by BD (1 mentions)
2 protocols using fitc conjugated mouse anti human cd90
1
Immunophenotyping of Dental Pulp Stem Cells
2
Flow Cytometric Characterization of HDFCs
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For flow cytometry analysis, HDFCs were harvested at passage 2, 106 suspended cells were washed and stained by incubation with the following antibodies and corresponding isotype controls: FITC-conjugated mouse antihuman CD34 (BD Biosciences, San Diego, CA), FITC-conjugated mouse antihuman CD45 (BD Biosciences), FITC-conjugated mouse antihuman CD90 (BD Biosciences) and FITC-conjugated mouse antihuman CD105 (BD Biosciences), and after 30 min of incubation and washing twice with PBS subsequently centrifuged for 5 min at 250g, and cells were resuspended in 500 mL PBS and filtered through a 100-μm strainer. Samples and controls were measured by a BD Flow Cytometer (BD Biosciences), and data analysed using FlowJo 7.6.1 software.
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