Teklad lm 485 mouse rat sterilizable diet 7012 chow

Naïve adult male C57BL/6 mice obtained from our in-house breeding colony were used for all experiments. Previously we found no sex differences in the antidepressant-like effect of desipramine or the SSRI escitalopram in the tail suspension test, therefore only male mice were used (Mitchell et al., 2013 (link)). Experiments were conducted in mice between 3 and 10 months of age. Animals were housed in a temperature-controlled (24 °C) vivarium maintained on a 12/12-hr light/dark cycle (lights on at 7:00 a.m.) in plastic cages (29 cm × 18 cm × 13 cm) containing 7090 Teklad sani-chip bedding (Envigo, East Millstone, NJ). Animals were given free access to water and Teklad LM-485 mouse/rat sterilizable diet 7012 chow (Envigo, East Millstone, NJ). Mice were weaned at P21 and housed in groups of no more than five same sex peers. All procedures were conducted in accordance with the National Institute of Health Guide for the Care and Use of Laboratory Animals (Institute of Laboratory Animal, Resources, Commission of Life Sciences, National Research Council, https://grants.nih.gov/grants/olaw/Guide-for-the-Care-and-use-of-laboratory-animals.pdf), and with the approval of the Institutional Animal Care and Use Committee, The University of Texas Health San Antonio (approval number: 20090119).

Mice with targeted disruption of Slc29a4 (PMAT) were generously donated by Dr. Joanne Wang (Duan & Wang, 2013 (link)). Thereafter, all mice were bred in house and maintained on a C57BL/6J background. Mice were housed in a temperature-controlled vivarium maintained at 24°C, on 7090 Teklad sani-chip bedding (Envigo, East Millstone, NJ), and given Teklad LM-485 mouse/rat sterilizable diet 7012 chow (Envigo) and water ad libitum. After weaning, mice were group housed with same-sex littermates at 2-5 mice per cage. Adult (≥90 days of age) male and female mice with PMAT alleles intact (+/+), reduced (+/−), or knocked out (−/−) were used for all experiments. Animal numbers ranged from 9-25 per sex per genotype; exact Ns for each measure are provided in corresponding figure legends. Food and water were provided ad libitum, and mice were housed in a 12:12 light:dark cycle with lights on at 0800 h. No procedures involved pain, and every effort was made to minimize the discomfort of the animals. All experiments were approved by the University of Texas Health Science Center at San Antonio Institutional Animal Care and Use Committee, and complied with the National Research Council’s Guide for the Care and Use of Laboratory Animals, 8^th Ed.