All chemicals were of analytical reagent grade and used without further purification. Double deionized (DI) water (18.6 MΩ) was used throughout the research work. The thiol terminal aptamer was purchased from Nzytech (Lisboa, Portugal) with a sequence SH-(CH2)6−CAG CAC CGA CCT TGT GCT TTG GGA GTG CTG GTC CAA GGG CGT TAA TGG ACA-3′ [14 (link)]. Syndrome coronavirus-2 receptor-binding domain (SARS-CoV-2-RBD), human serum albumin (HSA), human immunoglobulin A (HIgA), human immunoglobulin M (HIgM), human immunoglobulin G (HIgG), hemagglutinin (HA), neuraminidase (N), sodium tetrachloroaurate (NaAuCl4), sodium borohydride (NaBH4), phosphoric acid (H3PO4), potassium hydroxide (KOH), potassium ferricyanide (K3Fe(CN)6), potassium ferrocyanide (K4Fe(CN)6), chitosan, and hexaammineruthenium (III) chloride ([Ru(NH3)6]Cl3) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dual working screen-printed electrodes (Ref X1110) were obtained from Metrohm-Drop Sens (Llanera, Spain).
Thiol terminal aptamer
Manufactured by NZYTech
Sourced in Portugal
The Thiol terminal aptamer is a nucleic acid-based molecular recognition element designed to specifically bind to target molecules. It features a thiol group at its terminal end, which allows for convenient immobilization and integration into various analytical platforms.
Automatically generated - may contain errors
Lab products found in correlation
Hemagglutinin, by Merck Group (1 mentions)
Human serum albumin (hsa), by Merck Group (1 mentions)
Human immunoglobulin g, by Merck Group (1 mentions)
Hexaammineruthenium 3 chloride ru nh3 6cl3, by Merck Group (1 mentions)
Magnesium chloride mgcl2, by Thermo Fisher Scientific (1 mentions)
Neuraminidase na, by Merck Group (1 mentions)
2 protocols using thiol terminal aptamer
1
SARS-CoV-2 RBD Detection Aptamer Assay
2
Aptamer-Functionalized Gold Nanoparticles for SARS-CoV-2 Detection
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All chemicals were of analytical reagent grade and used without further purification. Double deionized (DI) water (18.6 MΩ) was used throughout the research work. Aluminium (Al) discs of 15 mm diameter were obtained from Good fellow. The thiol terminal aptamer was purchased from the Nzytech company and its sequence was: SH-(CH2)6-5′-CAG CAC CGA CCT TGT GCT TTG GGA GTG CTG GTC CAA GGG CGT TAA TGG ACA-3′ [52] (link). Gold (III) acetate Au (III) acetate, sodium borohydride (NaBH4), phosphoric acid (H3PO4), potassium hydroxide (KOH), 3-mercaptopropyl trimethoxysilane (3-MPS), oxalic acid (Ox), perchloric acid (HClO4), hydrogen peroxide (H2O2), and chromium (VI) oxide (H2CrO4), potassium ferrocyanide (Fe(CN)64−), and magnesium chloride (MgCl2) were obtained from Alfa Aesar. 3-mercapto-1-propanol (3-MCP), Tris (2-carboxyethyl) phosphine hydrochloride (TCEP), syndrome coronavirus-2 receptor-binding domain (SARS-CoV-2), hemagglutinin (HA) and neuraminidase (NA) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Kapton tape (Polyimide, 100 mm wide, 33 m long) was purchased from Satkit (Tarragona, Spain).
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