Anti o4 magnetic microbeads

Manufactured by Miltenyi Biotec

Anti-O4 magnetic microbeads are a laboratory product designed for cell isolation and separation. The microbeads are coated with antibodies specific to the O4 antigen, which is present on the surface of certain cell types. These beads can be used to magnetically label and isolate the corresponding cells from complex biological samples.

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Lab products found in correlation

Thyroid hormone, by Merck Group (1 mentions) Neural tissue dissociation kit, by Miltenyi Biotec (1 mentions) Neural basal media, by Thermo Fisher Scientific (1 mentions) Mouse multiple sclerosis rt2 profiler pcr array, by Qiagen (1 mentions) Ls column, by Miltenyi Biotec (1 mentions) Lightcycler 480, by Roche (1 mentions) Mirneasy kit, by Qiagen (1 mentions) Debris removal solution, by Miltenyi Biotec (1 mentions)

2 protocols using anti o4 magnetic microbeads

Isolation and Culture of O4+ OPCs

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Cortices from P6-P7 pups (seven pooled biological replicates per genotype, mixed sex) were dissected and subsequently dissociated with the Neural Tissue Dissociation Kit from Miltenyi. Anti-O4 magnetic microbeads (Miltenyi) were then used to isolate a pure population of O4+ OPCs. For culture and differentiation, serum- and antibiotic-free medium comprising 2% B27 (Gibco) and 30 ng/ml thyroid hormone (Sigma) in Neural Basal Media (Gibco) was used. Medium was changed daily and cells were harvested after 7 days.

Teo R.T., Hong X., Yu-Taeger L., Huang Y., Tan L.J., Xie Y., To X.V., Guo L., Rajendran R., Novati A., Calaminus C., Riess O., Hayden M.R., Nguyen H.P., Chuang K.H, & Pouladi M.A. (2016). Structural and molecular myelination deficits occur prior to neuronal loss in the YAC128 and BACHD models of Huntington disease. Human Molecular Genetics, 25(13), 2621-2632.

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Profiling Oligodendrocyte Response in Multiple Sclerosis

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Spinal cords were harvested in cold FIBSS and dissociated with the Adult Neural Dissociation Kit (Miltenyi). Single-cell suspensions were cleared from debris using the Debris Removal Solution (Miltenyi). To select for cells of the oligodendrocyte lineage, suspensions were incubated with 10 μl of anti-PDGFRα plus 10 μl of anti-O4 magnetic microbeads (Miltenyi) for 15 min at 4°C, then positively selected with LS columns (Miltenyi). Total RNA was isolated with a miRNeasy Kit (Qiagen), and gene expression assayed with the Mouse Multiple Sclerosis RT² Profiler PCR Array (Qiagen). Arrays were run on a LightCycler 480 real-time PCR machine (Roche). Relative expression was calculated with the ΔΔCt method (Livak and Schmittgen, 2001 (link)) after normalization to the housekeeping gene hsp90 and expressed as log2 fold change (FC) between groups.

Madsen P.M., Desu H.L., Vaccari J.P., Florimon Y., Ellman D.G., Keane R.W., Clausen B.H., Lambertsen K.L, & Brambilla R. (2019). Oligodendrocytes modulate the immune-inflammatory response in EAE via TNFR2 signaling. Brain, behavior, and immunity, 84, 132-146.

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