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Fluorescein isothiocyanate conjugated fitc anti human annexin 5 apoptosis detection kit 1

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The Fluorescein isothiocyanate-conjugated (FITC) anti-human Annexin V Apoptosis Detection Kit I is a laboratory instrument used to detect and quantify apoptosis, a programmed cell death process. The kit utilizes FITC-labeled Annexin V, a protein that binds to phosphatidylserine, a molecule that is exposed on the surface of apoptotic cells. This allows for the identification and measurement of apoptotic cells.

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Lab products found in correlation

Diva program, by BD (2 mentions) Propidium iodide, by BD (2 mentions) Facsaria 2 cell sorter system, by BD (1 mentions)

Close spelling variants of this product

Fluorescein isothiocyanate (FITC)-conjugated Annexin V Apoptosis Detection kit Annexin V-fluorescein isothiocyanate (FITC) Apoptosis Detection Kit I Fluorescein isothiocyanate-conjugated annexin V apoptosis detection kit I Annexin V-fluorescein isothiocyanate (annexin V-FITC) Apoptosis Detection Kit Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit Annexin-V-fluorescein-5-isothiocyanate (Annexin-V-FITC) apoptosis detection kit Annexin V-fluorescein isothiocyanate (FITC) apoptosis kit-I Annexin V-fluorescein isothiocyanate apoptosis detection kit I Annexin V fluorescein (FITC) Apoptosis Detection kit I Annexin V-fluorescein isothiocyanate (FITC) apoptosis kit

2 protocols using fluorescein isothiocyanate conjugated fitc anti human annexin 5 apoptosis detection kit 1

1

Apoptosis Detection with Flow Cytometry

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Propidium iodide (BD Bioscience) and the fluorescein isothiocyanate-conjugated (FITC) anti-human Annexin V Apoptosis Detection Kit I (BD Pharmingen) were used to characterize cells. Labeled cells (1 × 106) were analyzed using the BD FACS Aria II Cell Sorter System (BD Biosciences), followed by data analysis using the Diva program (BD Biosciences).
Ohta K., Hoshino H., Wang J., Ono S., Iida Y., Hata K., Huang S.K., Colquhoun S, & Hoon D.S. (2014). MicroRNA-93 activates c-Met/PI3K/Akt pathway activity in hepatocellular carcinoma by directly inhibiting PTEN and CDKN1A. Oncotarget, 6(5), 3211-3224.
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2

Apoptosis Quantification by Flow Cytometry

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A total of 5 × 105 cells were harvested and centrifuged for apoptotic evaluation. Propidium iodide (BD Bioscience) and the fluorescein isothiocyanate-conjugated (FITC) anti-human Annexin V Apoptosis Detection Kit I (BD Pharmingen) was used to characterize cells according to the manufacturer’s instructions. Labeled cells were detected using the fluorescence activated cell sorting (FACS) Aria II Cell Sorter System (BD Biosciences), followed by data analysis using the Diva program (BD Biosciences). Three independent experiments were done.
Li Y., Li Y., Chen Y., Xie Q., Dong N., Gao Y., Deng H., Lu C, & Wang S. (2017). MicroRNA-214-3p inhibits proliferation and cell cycle progression by targeting MELK in hepatocellular carcinoma and correlates cancer prognosis. Cancer Cell International, 17, 102.
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