The largest database of trusted experimental protocols

Tumor necrosis factor (tnf)

Manufactured by Mesoscale
Sourced in United States

The TNF is a laboratory instrument designed for the measurement and analysis of tumor necrosis factor (TNF) levels in various biological samples. It provides a reliable and efficient method for researchers to quantify TNF concentrations, which is a key cytokine involved in various cellular processes and immune responses.

Automatically generated - may contain errors

3 protocols using tumor necrosis factor (tnf)

1

Serum Cytokine and Antibody Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood was collected from CO2 euthanized mice via cardiac puncture, centrifuged (15 minutes, 10.000xg, 25°C), and serum was kept at -20°C. Serum levels of IFN-γ, IL-6, IL-10, IL-12p70, TNF and KC (CXCL1) were determined using the V-PLEX Custom Mouse Cytokine assay (Meso Scale Discovery, Maryland, USA). Serum MIF levels were measured by ELISA as recommended by the suppliers (R&D Systems). Alternatively, culture medium concentrations of MIF, TNF, CCL2 and KC (R&D Systems) as well as IFN-γ, IL-6 and IL-10 (Pharmingen) were determined by ELISA as recommended by the suppliers. Parasite-specific IgG responses were determined using soluble lysate freshly prepared from DEAE-purified T. congolense parasites recovered from WT mice at the peak of infection (around day 7). Lysate was coated overnight at 10 μg/ml PBS in 96-well Maxisorp plates (NUNC). Plates were washed (0.1% Tween 20 in PBS) and blocked (1% BSA in PBS) for 1 hour. Next, plates were washed and the sera (100 μl) serially diluted starting from 1/100 in blocking buffer were added. The ELISA was subsequently performed as described by the suppliers (SBA Clonotyping system-HRP kit (SouthernBiotech, USA)). As negative controls, blood samples incubated on lysate-free plates were used. The OD450nm recorded on lysate-free plates was subtracted from the OD450nm recorded on lysate-coated plates.
+ Open protocol
+ Expand
2

Intradermal LPS Administration Safety and Cytokine Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
Safety and tolerability were monitored by tracking adverse events, measuring vital signs, and standard laboratory tests (i.e. haematology) at 3, 6, 10, 24 and 48 hours after LPS administration. Circulating cytokines (IL‐1β, IL‐6, IL‐8, IL‐10, IFN‐γ and TNF; Meso Scale Discovery, Rockville, Maryland, USA) were measured in blood samples to detect a possible systemic effect of intradermal LPS administration. The blood samples for cytokine analysis were analysed in 2 batches with each having a different dilution, therefore the lower limit of quantitation (LLOQ) of the samples was the following: IL‐1β 0.298 or 0.745 pg/mL; IL‐6 1.52 or 3.81 pg/mL; IL‐8 1.25 or 3.12 pg/mL; IL‐10 0.702 or 1.76 pg/mL; IFN‐γ 10 or 25 pg/mL; and TNF 0.760 or 1.90 pg/mL.
+ Open protocol
+ Expand
3

Cytokine Release Profiling of Astrocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated at 5 × 104 cells per well in 96‐well plates coated with Matrigel. To control the number of cells, the assay was performed relatively soon after seeding. Therefore, the seeding density was higher than that in other assays or usual passages. Three days after plating, culture media were replaced with 100 μL/well of Astrocyte Media containing IL‐1β (Peprotech) or TNFα (Peprotech), harvested 24 h after replacement. Twenty‐five microlitres of conditioned media was subjected to electrochemiluminescence (ECL)‐ELISA analysis by using a V‐PLEX Custom Human Biomarkers kit (IL‐1β, IL‐6, TNF‐α, IL‐4 and GM‐CSF) (Meso Scale Discovery, Rockville, MD) and MESO SECTOR S600 (Meso Scale Discovery). Cytokine concentrations were calculated based on the kit's standard calibration curve using a four‐parameter logistic fit by applying MSD Discovery workbench 4.0 software (Meso Scale Discovery).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!