Cells were collected and lysed in lysis buffer (Cell Signaling Technology, Danvers, MA, USA) in the presence of complete protease-inhibitor mixture (Sigma-Aldrich). Protein concentration was determined by micro BCA assay (Pierce, Rockfod, IL, USA) and the indicated proteins were separated by SDS–PAGE and transferred onto polyvinylidene difluoride (PVDF, BioRad) or nitrocellulose membranes (Amersham Bioscience, Piscataway, NJ, USA). Membranes were probed using the following antibodies: anti-β-actin (1:10 000, Sigma-Aldrich); anti-calnexin (1:2000, Abcam, Cambridge, UK); anti-Tom20 (1:10000, Santa Cruz Biotechnology, Santa Cruz, CA, USA); anti- lactate dehydrogenase (LDH) (1:2000, Rockland); anti-long-chain fatty-acid CoA synthases (FACL4) (1:500, Santa Cruz Biotechnology); anti-calreticulin (CRT) (1:1000, Abcam); anti-c-FLIP Dave II clone (1:800, Alexis, Lausen, Switzerland); anti-RTN4 (1:1000, Abcam); anti-CLIMP63 (1:1000, Proteintech Group, Chicago, IL, USA); anti-cleaved caspase-3 (1:500, Cell Signaling Technology) and anti-PARP (1:1000; Cell Signaling Technology).
Anti calreticulin crt
Manufactured by Abcam
Sourced in Switzerland
Anti-calreticulin (CRT) is a laboratory reagent used to detect and quantify the presence of the calreticulin protein in various biological samples. Calreticulin is a multifunctional protein involved in calcium homeostasis, protein folding, and other cellular processes. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and flow cytometry to study the expression and localization of calreticulin in cells and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Complete protease inhibitor mixture, by Merck Group (1 mentions)
Lysis buffer, by Cell Signaling Technology (1 mentions)
Anti tom20, by Santa Cruz Biotechnology (1 mentions)
Nitrocellulose membrane, by Cytiva (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti parp, by Cell Signaling Technology (1 mentions)
Polyvinylidene fluoride (pvdf), by Bio-Rad (1 mentions)
Anti calnexin, by Abcam (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Trypan blue dye, by Merck Group (1 mentions)
Alexa 647, by Thermo Fisher Scientific (1 mentions)
Anti hsp90, by Santa Cruz Biotechnology (1 mentions)
Anti sqstm1 p62, by Abcam (1 mentions)
C188 9, by Selleck Chemicals (1 mentions)
Hsp70, by Cell Signaling Technology (1 mentions)
Alexa 488, by Thermo Fisher Scientific (1 mentions)
Alexa 568, by Thermo Fisher Scientific (1 mentions)
Recombinant interleukin 6 il 6, by Thermo Fisher Scientific (1 mentions)
Enliten atp assay system bioluminescence detection kit for atp measurement, by Promega (1 mentions)
Anti stat3, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti calreticulin crt
1
Western Blot Analysis of Cellular Proteins
2
Antibody Sources and Reagents for Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Anti-calreticulin (CRT) and anti-p62/SQSTM1 antibodies were purchased from Abcam. Anti-HN, anti-HSP90, and anti-STAT3 antibodies were obtained from Santa Cruz. Anti-β-actin and goat anti-rabbit antibody were purchased from Proteintech. Goat anti-mouse and Goat anti-rabbit antibodies for immunoblot analysis were obtained from Bioworld. The secondary antibodies of Alexa 488, Alexa 568 and Alexa 647 for immunofluorescence were obtained from Invitrogen. The following antibodies from Cell Signaling Technology were used: HMGB1, HSP70, poly (ADP-ribose) polymerase (PARP), p-eIF2α, p-STAT3 (Y705), Bcl-xl, Mcl-1, β-catenin. Mitoxantrone (MTX), Necrostain-1 (Nec-1), Z-VAD-FMK (Z-VAD), chloroquine (CQ), GSK2606414 (GSK), and C188-9 were obtained from Selleckchem. Recombinant interleukin-6 (IL-6) were obtained from PeproTech. Drugs were dissolved in dimethyl sulfoxide (DMSO) as stock solutions and stored at −20°C. ENLITEN®ATP Assay System Bioluminescence Detection Kit for ATP Measurement (#FF2000) was purchased from Promega. PI, Pierce®Protein Concentrator 2–6 mL/10K filters were purchased from Thermo Scientific. HMGB1 ELISA Kit II (#L534) was purchased from SHINO-TEST CORPORATION. Trypan blue dye was obtained from Sigma.
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