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Superose 12 gl column

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The Superose 12 GL column is a size exclusion chromatography column designed for the separation and purification of biomolecules such as proteins, peptides, and nucleic acids. The column features a prepacked matrix of highly cross-linked agarose beads, which provide a porous structure for the separation of molecules based on their size and molecular weight.

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Lab products found in correlation

Centrifuge 5415c, by Eppendorf (4 mentions) Kta purifier 900 series, by GE Healthcare (3 mentions)

Spelling variants of this product

Superose 12 10/300 GL column (80 citations) Superose 12 column (40 citations) Superose 12 (15 citations) Superose 12 10/300 GL gel filtration column (5 citations) Superose™ 12 10/300 GL SEC column (2 citations)

5 protocols using superose 12 gl column

1

Size-Exclusion HPLC Analysis

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Example 17

SE-HPLC was performed using an AKTA Purifier “900-series” (GE Healthcare). The system was equipped with a Superose 12 GL column (GE Healthcare, TC10/30) which was run at a constant flow rate of 0.3 mL per minute at room temperature. As running buffer 20 mM Tris, 100 mM sodium acetate, 500 mM sodium chloride, pH 7.4 was used. The sample was centrifuged (Centrifuge 5415C, Eppendorf, Vienna, Austria) for 5 min at 10,000 rpm and 100 μL were applied automatically by an autosampler. The absorbance of the column effluent was measured continuously at 280 nm.

US10758599B2. Stabilized liquid and lyophilized ADAMTS13 formulations (2020-09-01). BAXALTA INCORPORATED [US], BAXALTA GMBH [CH]. Inventors: Peter Matthiessen [AT], Peter L. Turecek [AT], Hans-Peter Schwarz [AT].
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2

SEC-HPLC Protein Purification Protocol

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Example 17

SE-HPLC was performed using an ÄKTA Purifier “900-series” (GE Healthcare). The system was equipped with a Superose 12 GL column (GE Healthcare, TC10/30) which was run at a constant flow rate of 0.3 mL per minute at room temperature. As running buffer 20 mM Tris, 100 mM sodium acetate, 500 mM sodium chloride, pH 7.4 was used. The sample was centrifuged (Centrifuge 5415C, Eppendorf, Vienna, Austria) for 5 min at 10,000 rpm and 100 μL were applied automatically by an autosampler. The absorbance of the column effluent was measured continuously at 280 nm.

US11564979B2. Stabilized liquid and lyophilized ADAMTS13 formulations (2023-01-31). TAKEDA PHARMACEUTICAL COMPANY LIMITED [JP]. Inventors: Peter Matthiessen [AT], Peter L. Turecek [AT], Hans-Peter Schwarz [AT].
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3

Size estimation of His6.TssA1B Nt1 (1–255)

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Size estimation of His6.TssA1B Nt1 (1–255) was performed on a Superose 12 GL column (GE Healthcare) in 50 mM Tris-Cl (pH 8.0) containing 500 mM NaCl. The following standards were used (molecular weights in kDa): thyroglobulin, 669; apoferritin, 443; alcohol dehydrogenase, 149.5; conalbumin, 75; ovalbumin, 43; carbonic anhydrase, 29; ribonuclease, 13.7.
Dix S.R., Owen H.J., Sun R., Ahmad A., Shastri S., Spiewak H.L., Mosby D.J., Harris M.J., Batters S.L., Brooker T.A., Tzokov S.B., Sedelnikova S.E., Baker P.J., Bullough P.A., Rice D.W, & Thomas M.S. (2018). Structural insights into the function of type VI secretion system TssA subunits. Nature Communications, 9, 4765.
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4

SEC-HPLC Protein Purification Protocol

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Example 17

SE-HPLC was performed using an ÄKTA Purifier “900-series” (GE Healthcare). The system was equipped with a Superose 12 GL column (GE Healthcare, TC10/30) which was run at a constant flow rate of 0.3 mL per minute at room temperature. As running buffer 20 mM Tris, 100 mM sodium acetate, 500 mM sodium chloride, pH 7.4 was used. The sample was centrifuged (Centrifuge 5415C, Eppendorf, Vienna, Austria) for 5 min at 10,000 rpm and 100 μL were applied automatically by an autosampler. The absorbance of the column effluent was measured continuously at 280 nm.

US10238720B2. Stabilized liquid and lyophilized ADAMTS13 formulations (2019-03-26). BAXALTA INCORPORATED [US], BAXALTA GMBH [CH]. Inventors: Peter Matthiessen [AT], Peter L. Turecek [AT], Hans-Peter Schwarz [AT].
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5

SEC-HPLC Protein Purification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 17

SE-HPLC was performed using an ÄKTA Purifier “900-series” (GE Healthcare). The system was equipped with a Superose 12 GL column (GE Healthcare, TC10/30) which was run at a constant flow rate of 0.3 mL per minute at room temperature. As running buffer 20 mM Tris, 100 mM sodium acetate, 500 mM sodium chloride, pH 7.4 was used. The sample was centrifuged (Centrifuge 5415C, Eppendorf, Vienna, Austria) for 5 min at 10,000 rpm and 100 μL were applied automatically by an autosampler. The absorbance of the column effluent was measured continuously at 280 nm.

US09937244B2. Stabilized liquid and lyophilized ADAMTS13 formulations (2018-04-10). BAXALTA INCORPORATED [US], BAXALTA GMBH [CH]. Inventors: Peter Matthiessen [AT], Peter L. Turecek [AT], Hans-Peter Schwarz [AT].
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