HCAECs were obtained from Cell Applications, Inc (USA). These cells were maintained in endothelial basal medium-2 (EBM-2; Lonza, cat. no. CC-3156) supplemented with 10% fetal bovine serum (FBS; GIBCO, Grand Island, NY, USA), 100 units/ml penicillin (GIBCO), 100 μg/ml streptomycin (GIBCO), and endothelial growth supplement (BD Biosciences). The cultures were maintained in a humidified incubator containing 5% CO2 at 37°C.
Endothelial growth supplement
Manufactured by BD
Sourced in United States
The Endothelial Growth Supplement is a laboratory reagent designed to support the growth and maintenance of endothelial cells in cell culture. It contains a proprietary blend of growth factors, hormones, and other nutrients essential for the proliferation and survival of endothelial cells.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Hcaec, by Cell Applications (1 mentions)
Ebm 2, by Lonza (1 mentions)
Mcdb105 medium, by Merck Group (1 mentions)
Potassium chloride (kcl), by Thermo Fisher Scientific (1 mentions)
Glycine, by Thermo Fisher Scientific (1 mentions)
Heparin, by Merck Group (1 mentions)
Normocin, by Thermo Fisher Scientific (1 mentions)
Endothelial cell growth medium, by Cell Applications (1 mentions)
2 protocols using endothelial growth supplement
1
Culturing Human Endothelial Cells
2
Culturing Primary Human Endothelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human umbilical vein endothelial cells (HUVECs) from single donors were purchased and cultured in endothelial cell growth medium (Cell Applications, San Diego, CA, USA). Early passage (1–3), single donor HUVECs were used for all experiments. Pulmonary Arterial Endothelial Cells (PAEC; passage 6 for Donor 1 and passage 7 for Donor 2) from donors without history of pulmonary hypertension were obtained from the Cleveland Clinic’s Biorepository [41 (link)]. PAEC were grown in MCDB105 medium (Sigma, Burlington, MA, USA) supplemented with 90 µg/mL heparin (Sigma, Burlington, MA), 90 µg/mL endothelial growth supplement (BD Biosciences, San Jose, CA, USA), 15 µg/mL glycine, 15 µg/mL KCl, 15% heat inactivated fetal bovine serum and normocin (Invitrogen, San Diego, CA, USA) as an antibacterial-antimycotic-antimycoplasmic agent.
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