Fixed tissues were embedded in Super Cryoembedding Medium-L1 (Leica Microsystems Japan, Tokyo, Japan) and serially sectioned at a 10 μm thickness with a CryoStar NX70 cryostat (Thermo Fisher Scientific Inc., Waltham, MA, USA) at ‒18°C. The sections were placed onto FRONTIER-coated slides (FRC-04; Matsunami Glass Ind., Ltd., Osaka, Japan), and then mounted with Fluoromount (Diagnostic BioSystems, Pleasanton, CA, USA). Confocal microscope with 10×, 20×, or 40× objective (FLUOVIEW FV1000; Olympus) was used for high magnification observation.
Super cryoembedding medium l1
Manufactured by Leica
Sourced in Japan
Super Cryoembedding Medium-L1 is a specialized medium used for cryosectioning of tissue samples. It is designed to provide a stable and supportive environment for the tissue during the freezing process, enabling thin and uniform sections to be obtained.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using super cryoembedding medium l1
1
Cryoembedding and Cryosectioning Protocol
2
Cryosectioning Tissues for Microscopy
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Fixed tissues were soaked in refrigerated sucrose solution (30% sucrose in PBS) until they sank. The tissues were then embedded in Super Cryoembedding Medium-L1 (Leica Microsystems Japan, Tokyo, Japan) and sectioned at 14- to 16-μm thickness using a CryoStar NX70 cryostat (Thermo Fisher Scientific Inc., Waltham, MA, USA) at −20 °C. The sections were placed onto FRONTIER-coated slides (FRC-04; Matsunami Glass Ind., Ltd., Osaka, Japan) and then mounted using Fluoromount (Diagnostic BioSystems, Pleasanton, CA, USA). A confocal microscope with 20× or 40× objective (FLUOVIEW FV1000; Olympus) was used for observation.
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