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Prolong gold with antifade

Manufactured by Thermo Fisher Scientific
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ProLong Gold™ with Antifade is a mounting medium designed for preserving fluorescence in microscopy samples. It contains an antifade reagent to prevent photobleaching of fluorescent labels.

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Close spelling variants of this product

ProLong Gold Antifade Mountant with DAPI reagent ProLongTM Gold Antifade Mountant with DAPI for nuclear staining ProLong Gold Antifade Mountant with 4′,6-diamidino-2-phenylindole (DAPI) ProLong™ Gold Antifade Mountant with DAPI for nuclear labeling ProLong gold antifade mounting medium with 4′,6-diamidino-2-phenylindole (DAPI; ProLong Gold antifade mounting media with DAPI ProLong Gold Antifade reagent with 4’,6-diamino-2-phenylindole (DAPI; ProLong Gold antifade reagent with 6-diamidino-2-phenylindole (DAPI) ProLong Gold antifade reagent with DAPI stain ProLong Gold Antifade Mountant with DAPI

2 protocols using prolong gold with antifade

1

Pimonidazole Labeling of Hypoxic Tumors

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One hour prior to sacrifice, tumour-bearing mice were injected with 60 mg/kg pimonidazole hydrochloride (Hypoxyprobe™-1 HPI, Inc.) diluted in ddH2O to a final concentration of 10 mg/mL) intravenously via the tail vein. Tumours were snap frozen immediately after cervical dislocation. Five-micrometre cryosections were thawed, rehydrated and fixed for 10 min in −20 °C acetone. Sections were incubated with 1:50 anti-pimonidazole overnight at 4 °C. Sections were then washed and mounted with ProLong Gold™ with Antifade (Invitrogen). Images were taken with an Axioplan Zeiss microscope.
Demircioglu F., Wang J., Candido J., Costa A.S., Casado P., de Luxan Delgado B., Reynolds L.E., Gomez-Escudero J., Newport E., Rajeeve V., Baker A.M., Roy-Luzarraga M., Graham T.A., Foster J., Wang Y., Campbell J.J., Singh R., Zhang P., Schall T.J., Balkwill F.R., Sosabowski J., Cutillas P.R., Frezza C., Sancho P, & Hodivala-Dilke K. (2020). Cancer associated fibroblast FAK regulates malignant cell metabolism. Nature Communications, 11, 1290.
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2

Hypoxia Visualization in Tumour Mice

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One hour prior to sacrifice, tumour-bearing mice were injected with 60 mg/kg pimonidazole hydrochloride (Hypoxyprobe™-1 HPI, Inc.) diluted in ddH2O to a final concentration of 10 mg/mL) intravenously via the tail vein. Tumours were snap frozen immediately after cervical dislocation. Five-micrometre cryosections were thawed, rehydrated and fixed for 10 min in −20 °C acetone. Sections were incubated with 1:50 anti-pimonidazole overnight at 4 °C. Sections were then washed and mounted with ProLong Gold™ with Antifade (Invitrogen). Images were taken with an Axioplan Zeiss microscope.
Demircioglu F., Wang J., Candido J., Costa A.S., Casado P., de Luxan Delgado B., Reynolds L.E., Gomez-Escudero J., Newport E., Rajeeve V., Baker A.M., Roy-Luzarraga M., Graham T.A., Foster J., Wang Y., Campbell J.J., Singh R., Zhang P., Schall T.J., Balkwill F.R., Sosabowski J., Cutillas P.R., Frezza C., Sancho P, & Hodivala-Dilke K. (2020). Cancer associated fibroblast FAK regulates malignant cell metabolism. Nature Communications, 11, 1290.
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