Ec 150 2 nucleodur 100 3 c18ec column

LC-MS/MS analysis was performed on a Thermo Finnigan (Villebon sur Yvette, France) LCQ Advantage ion trap mass spectrometer with an Electrospray Ionisation (ESI) source coupled to a Thermo Scientific Accela HPLC system (MS pump plus, autosampler, and PDA detector plus) with an EC 150/2 Nucleodur 100-3 C18ec column (Macherey-Nagel, Hoerdt, France). A gradient of water and acetonitrile (ACN) was applied from 5% to 30% ACN in 60 min and from 30% to 90% ACN in another 60 min at 30 °C. The flow rate was 0.3 mL/min. The injection volume was about 25 µL. All samples were measured in the positive and negative mode. The MS was operated with a capillary voltage of 10 V, source temperature of 240 °C, and high purity nitrogen as a sheath and auxiliary gas at a flow rate of 70 and 10 (arbitrary units), respectively. The ion was detected in a mass range of 50–2000 m/z, and collision energy of 35% was used in MS/MS for fragmentation. Data acquisitions were executed by Xcalibur 2.0.7 (Thermo Scientific, Illkirch-Graffenstaden, France).

LC-MS/MS analysis was performed on a Thermo Finnigan LCQ Advantage ion trap mass spectrometer with an ESI source coupled to a Thermo Scientific Accela HPLC system (MS pump plus, autosampler, and PDA detector plus) with an EC 150/2 Nucleodur 100-3 C18ec column (Macherey-Nagel). A gradient of water and acetonitrile (ACN) was applied from 5% to 30% ACN in 60 min. The flow rate was 0.3 mL/min. The MS was operated in the negative mode with a capillary voltage of 10 V, source temperature of 240 °C, and high purity nitrogen as a sheath and auxiliary gas at a flow rate of 70 and 10 (arbitrary units), respectively. Data acquisitions were executed by Xcalibur^TM 2.0.7 software (Thermo Scientific). The ions were detected in a mass range of 50–2000 m/z.