Linseeds were procured from Pansari inn, Pakistan. Linseeds were de-dusted and cleaned from physical impurities, and stored in an airtight container at ambient temperature. Microcrystalline cellulose (Avicel® PH 102) and HPMC K100M were procured from Merck, Germany. Moxifloxacin used in this research was according to the specification of United States Pharmacopoeia (USP). The KCl, NaOH, n-hexane, potassium dihydrogen phosphate and HCl were purchased from Riedel-de Haën, Germany. Magnesium stearate, polyvinylpyrrolidone K30 (PVP K30), talcum, sodium bicarbonate and β-cyclodextrin were procured from Sigma-Aldrich, USA. Linseeds hydrogel (LSH) was separated from linseeds using a nylon mesh. Deionized water was used for the entire study.
Potassium dihydrogen phosphate
Manufactured by Honeywell
Sourced in Germany
Potassium dihydrogen phosphate is a chemical compound commonly used as a laboratory reagent. It is a white, crystalline solid with the chemical formula KH2PO4. The primary function of potassium dihydrogen phosphate is to serve as a buffering agent, helping to maintain a specific pH level in various applications and experiments.
Automatically generated - may contain errors
Lab products found in correlation
N hexane, by Honeywell (2 mentions)
Hydrochloric acid (hcl), by Honeywell (2 mentions)
Magnesium stearate, by Merck Group (1 mentions)
Potassium chloride (kcl), by Honeywell (1 mentions)
Avicel ph 102, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Honeywell (1 mentions)
β cyclodextrin, by Merck Group (1 mentions)
Sodium bicarbonate, by Merck Group (1 mentions)
Ethanol, by Honeywell (1 mentions)
Acetic acid, by Honeywell (1 mentions)
Gum tragacanth, by Merck Group (1 mentions)
Avicel ph 101, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Temed, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Potassium chloride (kcl), by Merck Group (1 mentions)
Disodium hydrogen phosphate, by Merck Group (1 mentions)
Yeast extract, by Solabia (1 mentions)
Nicotinamide adenine dinucleotide phosphate, by Merck Group (1 mentions)
Peptone, by Solabia (1 mentions)
7 protocols using potassium dihydrogen phosphate
1
Linseed-based Moxifloxacin Delivery System
2
Psyllium Husk-Theophylline Formulation Development
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Psyllium husk was procured from Marhaba Laboratories Pvt. Ltd., Lahore, Pakistan. Anhydrous theophylline (TP) was purchased from MP Biomedicals, Inc. Solon, Ohio, USA. Potassium dihydrogen phosphate (≥99.0%), sodium chloride (NaCl) (≥99.5%), potassium chloride (KCl) (≥99.5%), acetic acid (≥99.8%), hydrochloric acid (37.0%), ethanol (≥98.0%) and n-hexane (≥95.0%) were obtained from Riedel-de Haën, Germany. Sodium hydroxide (NaOH) (≥98.0%) was purchased from Merck Chemicals GmbH, Darmstadt, Germany. Microcrystalline cellulose (Avicel® PH 101) and tragacanth gum were procured from Sigma-Aldrich Co. (St. Louis, MO, USA). All reagents and chemicals were of analytical grade and used as such without further purification. Simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) were prepared as per procedure given in USP 34-NF 29. Deionized water (DW) was used throughout this study.
3
pH and Salt-Responsive Hydrogel Synthesis
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Hydroxyethyl methacrylate (HEMA), 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS), and 2-(diethylamino)ethyl methacrylate (DEAEMA) were provided by Merck. The chemical crosslinker diethyleneglycol dimethacrylate (DEGDMA, Fluka), the redox-initiator system ammonium persulfate (APS, Merck, Darmstadt, Germany), and N,N,N′,N′-tetramethylethylenediamine (TEMED, Merck) were used as received. Halloysite nanotube (HNT) was supplied by ESAN Eczacibasi, Turkey. The elemental composition of HNT was (wt.%): SiO2, 43.30; Al2O3, 38.40; Fe2O3, 0.80; TiO2, 0.10. Disodium hydrogen phosphate (Merck), potassium dihydrogen phosphate (Riedel-de Haen, Darmstadt, Germany), hydrochloric acid (Merck), and potassium phosphate (J.T. Baker, Phillipsburg, New Jersey, USA) were used for pH-responsive swelling experiments. Potassium iodide (KI, Carlo Erba, Sabadell, Barcelona), potassium bromide (KBr, Merck), and potassium chloride (KCl, Merck) were used for the salt-responsive swelling experiments. Deionized water was used for all the synthesis stages as well as for swelling measurements.
4
Biochemical Assays for Oxidative Stress
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All the following chemicals used in this study were of high quality. Yeast extract, Agar and peptone were received from BIOKAR Diagnostics. Tris (hydroxymethyl) aminomethane, Hydrogen peroxide (H2O2), Glycerol, Phenylmethylsulfonyluoride (PMSF), Guanidine hydrochloride and 2-β-mercaptoethanol were purchased from Fluka. Potassium persulfate (K2S2O8) from SOCHID. Vitamin C and TrichlorAcetic acid (TCA) were bought from Scharlau (Spain). Bovine serum albumin (BSA) was purchased from Janssen Chimica. Potassium dihydrogen phosphate, Potassium cyanide (KCN), EthylenediaminetetraAcetic acid (EDTA), Sodium dodecyl sulphate (SDS) and Sodium carbonate were bought from Riedel-de Haën. Ethanol was received from Biosmart. Acetic acid, 2,6- Dichlorophenolindophenol (DCIP), Succinate, oxidized glutathione (GSSG), Thiobarbituric acid (TBA), Sodium chloride (NaCl), Nicotinamide adenine dinucleotide (NADH), Nicotinamide adenine dinucleotide phosphate (NADPH), 2,4- dinitrophenylhydrazine (DNPH) reagent and Magnesium chloride (MgCl2) were purchased from Sigma-Aldrich.
5
Comprehensive Reagent Inventory for Antioxidant Assays
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The following reagents were purchased from Merck (Darmstadt, Germany): potassium persulfate (99%), sodium chloride (99.5%), disodium phosphate dodecahydrated (99%), glacial acetic acid (100%), sodium carbonate (p.a.), and ferrous sulfate heptahydrate (99%), from Fluka (Lisbon, Portugal), 2,2-diphenyl-1-picrylhydrazyl (DPPH) (>95%), Trolox (≥99.8%), 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) (≥99%), 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) (≥99.0%), β-Carotene (≥97%, UV), Tween 40 and Folin–Ciocalteu’s phenol reagent were purchased from Fluka (Lisbon, Portugal). Potassium chloride (>99.5%), gallic acid (99%), rutin (≥98%, HPLC) and ferric chloride hexahydrated (97%–102%) were purchased from Panreac (Barcelona, Spain); potassium dihydrogen phosphate (99.5%), aluminum chloride (98%), sodium acetate trihydrate (pure) and dimethylsulfoxide (97%) were purchased from Riedel-de Haën (Hanover, Germany). Alamar Blue solution was purchased from Trek Diagnostics (Westlake, OH, USA).
All solvents for plant extraction were AR grade, from Fisher (Lisbon, Portugal). Water was purified by a Milli-Q Gradient system (Millipore, Milford, MA, USA).
All solvents for plant extraction were AR grade, from Fisher (Lisbon, Portugal). Water was purified by a Milli-Q Gradient system (Millipore, Milford, MA, USA).
6
Analytical Method Development for Compounds
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ANZ, NFZ reference standard were provided by Schazoo Labs (Pakistan). Acetonitrile (ACN), potassium dihydrogen phosphate (KH 2 PO 4 ), phosphoric acid (H 3 PO 4 , 85%) and triethylamine (TEA) were supplied by Honeywell (USA). All the chemicals and reagents were of analytical grade while GenPure water system (Thermo Scientific, USA) was used to obtain ultrapure water (18 MΩ.cm -1 ).
7
Recombinant Insulin Formulation Protocol
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Recombinant human insulin (batch No. SPIN-05-101B) was provided by Novo Nordisk A/S. Zinc acetate (CAS No. 5970-45-6) was purchased from Sigma–Aldrich (now part of Merck KGaA). Sodium thiocyanate (CAS No. 540-72-7) was purchased from Fluka Chemie (now part of Merck KGaA). Disodium hydrogen phosphate (CAS No. 10028-24-7) was purchased from PanReac AppliChem ITW Reagents. Potassium dihydrogen phosphate (CAS No. 7778-77-0) was purchased from Honeywell International Inc. Deionized water from an Aquatron A4D system equipped with a Zalion 200.4 column was used in all experiments.
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