B3259

Manufactured by Merck Group

Sourced in France

B3259 is a laboratory equipment product from Merck Group. It is designed for general laboratory use. The core function of this product is to [DESCRIPTION NOT AVAILABLE].

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Lab products found in correlation

Dmem f12, by Thermo Fisher Scientific (1 mentions) Complete mini, by Roche (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Sh sy5y, by Thermo Fisher Scientific (1 mentions) Sh sy5y, by Merck Group (1 mentions) C4382, by Merck Group (1 mentions) M1404, by Merck Group (1 mentions) A0281, by Merck Group (1 mentions) Tune mix, by Agilent Technologies (1 mentions) C7752, by Merck Group (1 mentions) U6253, by Merck Group (1 mentions)

4 protocols using b3259

Stimulating IP3 Release in Cells

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To stimulate IP3 release and thus IP3R, cells were treated with bradykinin (fibroblasts) or carbachol (SH-SY5Y). Prior to stimulation, cells underwent serum starvation. For this, cells were first washed in PBS (Sigma) and then cultured for 4 hours in DMEM (fibroblasts; Thermo Fisher Scientific) or DMEM/F12 (SH-SY5Y; Thermo Fisher Scientific) without FBS. Afterwards cells were treated with 300 nm bradykinin (fibroblasts; B3259, Sigma, powder dissolved in ddH₂O) or 1 mm carbachol (SH-SY5Y; C4382, Sigma, dissolved in ddH₂O) in the respective culture medium for t = 0, 30, 60 min (fibroblasts) or t = 0, 2, 4 h (SH-SY5Y). After treatment, cells were washed in PBS and scraped in adioimmunoprecipitation assay (RIPA) buffer (Sigma) including protease inhibitor (cOMPLETE Mini, Roche Applied Sciences) (fibroblasts) or PBS (SH-SY5Y). Immunoblots were then performed as described above.

Wagner M., Osborn D.P., Gehweiler I., Nagel M., Ulmer U., Bakhtiari S., Amouri R., Boostani R., Hentati F., Hockley M.M., Hölbling B., Schwarzmayr T., Karimiani E.G., Kernstock C., Maroofian R., Müller-Felber W., Ozkan E., Padilla-Lopez S., Reich S., Reichbauer J., Darvish H., Shahmohammadibeni N., Tafakhori A., Vill K., Zuchner S., Kruer M.C., Winkelmann J., Jamshidi Y, & Schüle R. (2019). Bi-allelic variants in RNF170 are associated with hereditary spastic paraplegia. Nature Communications, 10, 4790.

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Bradykinin-induced Collagen Gel Contraction

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Tensin1 knockdown was performed as above and then cells were detached and embedded in collagen gels as described previously^{32 (link)}. Bradykinin was then added to appropriate wells to a final concentration of 1 nM (B3259, Sigma-Aldrich). Photographs were taken at 0, 4, 18, 24 and 48 hours. The surface area was measured at each time point using ImageJ software (National Institutes of Health; http://rsbweb.nih.gov/ij/).

Stylianou P., Clark K., Gooptu B., Smallwood D., Brightling C.E., Amrani Y., Roach K.M, & Bradding P. (2019). Tensin1 expression and function in chronic obstructive pulmonary disease. Scientific Reports, 9, 18942.

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Modulating MSC Adhesion to Hydrogels

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Lysophosphatidic acid (Enzo Life Sciences #BML-LP100), bradykinin (Sigma #B3259), nocodazole (Sigma #M1404), cytochalasin D (Sigma #C2618), and Rac inhibitor (Millipore #553502) were included in culture media at concentrations of 20 μM, 10 nM, 0.5 μM, 0.5 μM, and 50 μM respectively. Drugs were stored at 100x in DMSO at −20°C until use. To reduce MSC adhesion to the hydrogel substrate surface by blocking RGD peptide-binding integrins, 0.02, 0.05, 0.1 and 0.2 mM free RGD peptide was included in the culture media throughout the duration of the timelapse.

Wen J.H., Choi O., Taylor-Weiner H., Fuhrmann A., Karpiak J.V., Almutairi A, & Engler A.J. (2015). Haptotaxis is cell type specific and limited by substrate adhesiveness. Cellular and molecular bioengineering, 8(4), 530-542.

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Ion Mobility Peak Width Analysis

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Samples included a mixture of hexakis-(fluooalkyloxy)phosphazines (TuneMix, Agilent Technologies), bradykinin (B3259, ≥ 98%, Sigma-Aldrich, Saint Quentin-Fallavier, France), bovine ubiquitin (U6253, ≥ 98% , Sigma-Aldrich), equine cytochrome c (C7752, assay ≥ 95%, Sigma-Aldrich), bovine serum albumin (A0281, ≥ 99%, Sigma-Aldrich), deoxyribonucleic acids (RPcartridge-gold purity, Eurogentec, Seraing, Belgium) and an artificial foldamer NO2-QX7-O-X7Q-NO2 (provided by I. Huc, University of Bordeaux). 20 TuneMix was used as received. The foldamer was sprayed from 95/5 (v:v) CHCl3/MeOH. Native proteins were prepared in aqueous ammonium acetate (50-200 mM), and 0.5% sulfolane was added to ubiquitin to promote the formation of 7+ ions. The polythymine mixture was sprayed from RNAse free water. The higherorder nucleic acid structures (duplexes and quadruplexes) were sprayed from aqueous ammonium acetate (100 or 150 mM). The present article focuses on the data treatment, and the samples were selected mainly to illustrate different behaviors in terms of ion mobility peak width. More detail on sample preparation can be found in the supporting information.

Marchand A., Livet S., Rosu F, & Gabelica V. (2017). Drift Tube Ion Mobility: How to Reconstruct Collision Cross Section Distributions from Arrival Time Distributions?. Analytical chemistry, 89(23).

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