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Human antibody array 1000

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The Human Antibody Array 1000 is a comprehensive tool for the simultaneous detection and quantification of 1,000 different human antibodies in a single experiment. The array allows for the rapid and efficient profiling of the human antibody repertoire across a wide range of samples and conditions.

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Antibody arrays (3 citations) Human L-1000 Antibody Arrays (2 citations) Human Cytokine Antibody Arrays G-Series 1000 (2 citations)

2 protocols using human antibody array 1000

1

Plasma Protein Detection by Antibody Array

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Protein expression in human plasma was detected by the Human Antibody Array 1000 produced by RayBio (RayBiotech; Cat# AAH-BLG-1000; USA) in the discovery stage. It was performed according to the standard protocol provided by the manufacturer (https://www.raybiotech.com/files/manual/Antibody-Array/AAH-BLG-1000.pdf).
Tang Y., Yang K., Liu Q., Ma Y., Zhu H., Tang K., Geng C., Xie J., Zhuo D., Wu W., Jin L., Xiao W., Wang J., Zhu Q, & Liu J. (2023). Preosteoclast plays a pathogenic role in syndesmophyte formation of ankylosing spondylitis through the secreted PDGFB — GRB2/ERK/RUNX2 pathway. Arthritis Research & Therapy, 25, 194.
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2

Cytokine Profiling of Colon Cancer Cell Co-cultures

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The culture media in the upper (0.25 mL) and lower (0.75 mL) transwell chambers were harvested after the cells migrated for 24 hours. Approximately 1 mL of cell medium was collected from SW480, SW480/HHSECs, SW480/HUVECs, HCT116, HCT116/HHSECs, HCT116/HUVECs, HHSECs, and HUVECs. The collected supernatants were centrifuged at 1000 g for 10 min and the protein expression profiles were analyzed using Human Antibody Array 1000 (Raybiotech, AAH-BLG-1000, USA), which detects 1000 cytokines. The protocol was performed in accordance with the instructions of the manufacturer. Signal intensity was measured by spot densitometry using streptavidin-conjugated HiLyte Fluor 532™ for fluorescence detection. The complete array maps can be found at http://www.raybiotech.com/human-l-1000-array-glass-slide-2.html.
Hu C.T., Guo L.L., Feng N., Zhang L., Zhou N., Ma L.L., Shen L., Tong G.H., Yan Q.W., Zhu S.J., Bian X.W., Lai M.D., Deng Y.J, & Ding Y.Q. (2015). MIF, secreted by human hepatic sinusoidal endothelial cells, promotes chemotaxis and outgrowth of colorectal cancer in liver prometastasis. Oncotarget, 6(26), 22410-22423.
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