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Annexin 5 apc 7 aad staining kit

Manufactured by MultiSciences Biotech
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Sourced in China

The Annexin V-APC/7-AAD Staining Kit is a laboratory tool used for the detection and quantification of apoptotic cells. The kit utilizes Annexin V, a calcium-dependent phospholipid-binding protein, conjugated to the fluorescent dye APC (Allophycocyanin), and the DNA-binding dye 7-AAD (7-Aminoactinomycin D) to identify cells undergoing early and late stages of apoptosis.

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Lab products found in correlation

Cell cycle staining buffer, by MultiSciences Biotech (1 mentions) Facscanto, by BD (1 mentions)

Close spelling variants of this product

Annexin V-APC 7-AAD Annexin V

3 protocols using annexin 5 apc 7 aad staining kit

1

Apoptosis and Cell Cycle Assessment

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Apoptosis assays were performed after 72 h, and the cells were harvested according to the manufacturer’s instructions using an Annexin V-APC/7-AAD staining kit (MultiSciences, China). The apoptotic rate of the cells was determined by flow cytometry (BD FACSCanto, USA). All data were exported as FCS 3.0 documents and analyzed with FlowJo software 10.0.7. Cell cycle assays were performed after 24 h, and cells were harvested according to the manufacturer’s instructions for the cell cycle staining buffer (MultiSciences, China).
Zhang Q., Liu R.X., Chan K.W., Hu J., Zhang J., Wei L., Tan H., Yang X, & Liu H. (2019). Exosomal transfer of p-STAT3 promotes acquired 5-FU resistance in colorectal cancer cells. Journal of Experimental & Clinical Cancer Research : CR, 38, 320.
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2

Paclitaxel-induced Apoptosis Assay

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Cells were treated with paclitaxel for 24 hours and harvested by centrifugation. Then, according to the manufacturer's instructions, cell apoptosis assays were performed by FACS using an Annexin V-APC/7-AAD Staining Kit (Multi-Science(Lianke) Biotech Co.). All tests were performed independently three times.
Qiu N., Jin H., Cui L., Zhan Y.T., Xia H.M., Jiang M., Zhou J., Liang H.L., Ao X., He Z.M, & Li H.S. (2023). IFT20 Confers Paclitaxel Resistance by Triggering β-arrestin-1 to Modulate ASK1 Signaling in Breast Cancer. Molecular cancer research : MCR, 21(3).
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3

Paclitaxel-induced Apoptosis Assay

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Cells were treated with paclitaxel for 24 hours and harvested by centrifugation. Then, according to the manufacturer's instructions, cell apoptosis assays were performed by FACS using an Annexin V-APC/7-AAD Staining Kit (Multi-Science(Lianke) Biotech Co.). All tests were performed independently three times.
Qiu N., Jin H., Cui L., Zhan Y., Xia H., Jiang M., Zhou J., Liang H., Ao X., He Z., & Li H. (2021). IFT20 confers paclitaxel resistance by triggering β-arrestin-1 to modulate ASK1 signaling in breast cancer.
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