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Recombinant human secretin

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Recombinant human secretin is a laboratory-produced protein that is functionally and structurally identical to the naturally occurring human secretin hormone. Secretin is a peptide hormone primarily produced in the duodenum and is involved in the regulation of digestive processes.

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Lab products found in correlation

Lsm 510 microscope, by Zeiss (2 mentions) Cellmask orange plasma membrane stain, by Thermo Fisher Scientific (2 mentions)

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Secretin (7 citations)

2 protocols using recombinant human secretin

1

Secretin Induces Membrane Localization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Twenty day cholangioids were incubated with vehicle or 100 nM recombinant human secretin (Sigma-Aldrich) and stained with 1:1000 Cell MaskOrange Plasma membrane stain (Life Technologies, Carlsbad CA) for 15 min at 37°C, 5% CO2. After two washes with cholangioid media, live images where taken using a Zeiss LSM 510 microscope.
Loarca L., De Assuncao T.M., Jalan-Sakrikar N., Bronk S., Krishan A., Huang B., Morton L., Trussoni C., Bonilla L.M., Krueger E., O’Hara S., Splinter P., Shi G., Pisarello M.J., Gores G.J., Huebert R.C, & LaRusso N.F. (2017). Development and Characterization of Cholangioids from Normal and Diseased Human Cholangiocytes as an In Vitro Model to Study Primary Sclerosing Cholangitis. Laboratory investigation; a journal of technical methods and pathology, 97(11), 1385-1396.
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2

Secretin Induces Membrane Localization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Twenty day cholangioids were incubated with vehicle or 100 nM recombinant human secretin (Sigma-Aldrich) and stained with 1:1000 Cell MaskOrange Plasma membrane stain (Life Technologies, Carlsbad CA) for 15 min at 37°C, 5% CO2. After two washes with cholangioid media, live images where taken using a Zeiss LSM 510 microscope.
Loarca L., De Assuncao T.M., Jalan-Sakrikar N., Bronk S., Krishan A., Huang B., Morton L., Trussoni C., Bonilla L.M., Krueger E., O’Hara S., Splinter P., Shi G., Pisarello M.J., Gores G.J., Huebert R.C, & LaRusso N.F. (2017). Development and Characterization of Cholangioids from Normal and Diseased Human Cholangiocytes as an In Vitro Model to Study Primary Sclerosing Cholangitis. Laboratory investigation; a journal of technical methods and pathology, 97(11), 1385-1396.
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