Sc 57053

Manufactured by Santa Cruz Biotechnology

Sc-57053 is a laboratory product manufactured by Santa Cruz Biotechnology. It is a specialized piece of equipment designed for use in research and scientific applications. The core function of Sc-57053 is to facilitate specific tasks or processes within the laboratory environment.

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Lab products found in correlation

3 protocols using sc 57053

Multiplex Immunohistochemistry for Autophagy Markers

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Anti-TRPV1 (1:100, ab6166), anti-LC3 (1:1000, ab192890), anti-P62 (1:1000, ab109012), anti-LAMP2 (1:100, ab13524), anti-H2 Db/H2-D1(1:100, ab25244), and Alexa Fluor 568 (1:500), Alexa Fluor 488 (1:500) secondary antibody were purchased from Abcam. Anti-CGRP (1:100, sc-57053) was purchased from Santa cruze. Anti-mouse CD45-PE-cy7 (103114), anti-mouse CD11c-APC-cy7 (117323), anti-mouse CD3-APC-cy7 (100221), anti-mouse CD8a-APC (100711), anti-mouse CD16/32-TruStain FcX™ (101320), anti-mouse CD86-APC (105011) antibodies were purchased from BioLegend. APC anti-mouse H-2 (E-AB-F1216E) and Anti-mouse CD80-FITC (E-AB-F0992C) were purchased from Elabscience. HRP-conjugated anti-rabbit antibody (W4011) and HRP-conjugated anti-mouse antibody (W4021) were purchased from Promega. Enhanced chemiluminescence (ECL) kit was purchased from Biological Industries. One Step TUNEL Apoptosis Assay Kit (Green Fluorescence) (C1088) and Ad-GFP-LC3B (C3006) were purchased from Beyotime. Lyso-Tracker Red (L12492) was purchased from Invitrogen. Imiquimod (HY-B0180A) was purchased from MCE. Indocyanine Green (3599-32-4) was purchased from Aladdin. Morphine (161007-2) was purchased from NORTHEAST PHARM. Ropivacaine (R413090) was purchased from Mackline. Pluronic F127 (P2443) was purchased from Sigma.

Zhang J., Zhu S., Zhao M., Zhou M., Zhu X., Qing X., Yang Z., Wei P., Zhang G., He W., Yu Y, & Liu X. (2023). Analgesic and potentiated photothermal therapy with ropivacaine-loaded hydrogels. Theranostics, 13(7), 2226-2240.

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Analysis of Trigeminal Nerve Nucleus Markers

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On day 9, at 12 hours after administering NTG/VEH, the mice were deeply anesthetized with 1% pentobarbital sodium, and the brain TNC tissues of the medulla oblongata were quickly harvested on ice, frozen in liquid nitrogen, and stored at − 80 °C. The TNC tissues were sonicated in RIPA lysis buffer, added with 1 mM Na₃VO₄, 1 mM DTT, 1 mM PMSF, 20 mM NaF, and 2 mM protease inhibitor cocktail, and quantified using the Bradford method. Proteins were separated using 8%–12% SDS-PAGE gels and were transferred to the PVDF membrane (Millipore). The PVDF membranes were blocked with 5% milk for 2 hours at room temperature, and were incubated overnight at 4 °C with the following primary antibodies: anti-c-Fos (1:1000; ab222699 Abcam), anti-CGRP (1:200; sc-57,053 Santa Cruz), anti-VGluT1 antibody (1:1000; ab227805 Abcam) and anti-GAPDH (1:5000; YM3445 ImmunoWay). Then, the membranes were washed in TBST and incubated with goat anti-rabbit or anti-mouse secondary antibodies (1:1000; Boster) for 1 hour at room temperature. The bands were visualized with Chemiluminescent HRP substrate (Millipore) on Azure Biosystem C500 and quantified using ImageJ.

Wang L., Liu X., Zhu C., Wu S., Li Z., Jing L., Zhang Z., Jing Y, & Wang Y. (2024). Environmental enrichment alleviates hyperalgesia by modulating central sensitization in a nitroglycerin-induced chronic migraine model of mice. The Journal of Headache and Pain, 25(1), 74.

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Immunofluorescence Imaging of DRG Neurons

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Immunofluorescence was performed as described in previous reports.^{28 (link)} Briefly, L3-L4 DRGs were rapidly detached from mice perfused with 4% paraformaldehyde (BL539 A, Biosharp) and post-fixed with 4% paraformaldehyde for 2 h, followed by dehydration in 5%, 15%, 30% sucrose gradients, respectively, and sectioned at a thickness of 12 mm on a cryostat. After blocking at room temperature for 1 h and washing with PBST (containing 0.04% Triton), the slices were incubated with anti-rabbit ETS1 antibody (1:1000; A13302, ABclonal), mouse anti-glutamine synthetase (GS; 1:800; EM1902-39, Hua Bio), mouse anti-β-tubulin (1:800; M0805-8, Hua Bio), mouse anti-CGRP (1:50; SC57053, Santa Cruz), mouse anti-P2X3 (1:100; SC390572, Santa Cruz), or mouse anti-NF200 (1:100; 60,331-1-Ig, Proteintech) at 4°C overnight. After washing with PBST, slices were finally incubated with fluorescent-conjugated secondary antibodies Alexa Fluor^TM 488 goat anti-rabbit IgG (A21206, Molecular Probes) and Alexa Fluor™ 594 goat anti-mouse IgG (A10036, Molecular Probes). The sections were mounted on a mounting medium with DAPI (0100-20, Southern Biotech) and scanned using a high-resolution digital confocal microscope (Olympus FV1000, Japan).

Zheng H.L., Sun S.Y., Jin T., Zhang M., Zeng Y., Liu Q., Yang K., Wei R., Pan Z, & Lin F. (2023). Transcription factor ETS proto-oncogene 1 contributes to neuropathic pain by regulating histone deacetylase 1 in primary afferent neurons. Molecular Pain, 19, 17448069231152125.

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