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2 protocols using wbb6f1 kit w v

1

Examining Placental Angiogenesis in Mice

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Pregnant FVB/NJ mice, WBB6F1- Kit W− v/ + were obtained from Jackson Laboratories (Bal Harbor, ME). Primary antibodies utilized were: c-kit (1:50, DAKO Carpinteria, CA), SCF (1:500; Abcam, Cambridge, MA), α-Smooth Muscle Actin (α-sma: 1:500, Sigma-Aldrich; St. Louis, MO), von Willebrand factor (vWF: 1:200; DAKO), and Ki67 (1:100; Abcam). Secondary antibodies utilized were: Biotinylated anti-mouse IgG (1:200, Vector, CA), HRP Conjugated Donkey Anti-Goat IgG (1:2000, Jackson Immunoresearch, PA), Goat Anti-Mouse IgG Alkaline Phosphatase (1:100, Sigma-Aldrich), and Goat Anti-Rabbit IgG-Peroxidase (1:100, Sigma-Aldrich). Mouse c-kit neutralizing antibody (ACK2; 50μg/kg) and recombinant SCF were both obtained from EBioscience (San Diego, CA). FR180204 (a selective ERK1/2 antagonist) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA).
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2

Examining Placental Angiogenesis in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Pregnant FVB/NJ mice, WBB6F1- Kit W− v/ + were obtained from Jackson Laboratories (Bal Harbor, ME). Primary antibodies utilized were: c-kit (1:50, DAKO Carpinteria, CA), SCF (1:500; Abcam, Cambridge, MA), α-Smooth Muscle Actin (α-sma: 1:500, Sigma-Aldrich; St. Louis, MO), von Willebrand factor (vWF: 1:200; DAKO), and Ki67 (1:100; Abcam). Secondary antibodies utilized were: Biotinylated anti-mouse IgG (1:200, Vector, CA), HRP Conjugated Donkey Anti-Goat IgG (1:2000, Jackson Immunoresearch, PA), Goat Anti-Mouse IgG Alkaline Phosphatase (1:100, Sigma-Aldrich), and Goat Anti-Rabbit IgG-Peroxidase (1:100, Sigma-Aldrich). Mouse c-kit neutralizing antibody (ACK2; 50μg/kg) and recombinant SCF were both obtained from EBioscience (San Diego, CA). FR180204 (a selective ERK1/2 antagonist) was obtained from Santa Cruz Biotechnology (Santa Cruz, CA).
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