NMR spectra were obtained on a Bruker 400‐MHz Avance III spectrometer using a 5‐mm double resonance broad band probe (model BBO). Estrone was purchased from Steraloids, Inc. (Newport, RI) and used as received, and a proton NMR is included in the Supporting information . 2‐t‐ButylEstrone (7 ) was made by using the method of Liu et al.12 2‐(t‐butyl‐d9)Estrone was made by the same method by using t‐butyl alcohol‐d10 (98%, Cambridge Isotopes, Andover, MA). Proton and carbon spectra for both 2‐t‐butylEstrone and 2‐(t‐butyl‐d9)Estrone are included in the Supporting information . All other chemicals were obtain from Fisher Scientific Co. (Fair Lawn, NJ) or Aldrich Chemical Co. (Milwaukee, WI) and used as received.
Estrone
Manufactured by Steraloids
Sourced in United States
Estrone is a steroid hormone that belongs to the class of estrogens. It is a key component in the regulation of the menstrual cycle and the development of female secondary sexual characteristics. Estrone is a naturally occurring compound that can be extracted or synthesized for research and laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Equilenin, by Steraloids (1 mentions)
15n labeled nh4cl, by Cambridge Isotopes (1 mentions)
Pregnenolone, by Steraloids (1 mentions)
Testosterone, by Cerilliant (1 mentions)
Androstenedione, by Steraloids (1 mentions)
D9 progesterone p4 d9, by Steraloids (1 mentions)
D3 testosterone, by Cerilliant (1 mentions)
3 protocols using estrone
1
Synthesis and NMR Characterization of Estrone Derivatives
2
Steroid Compound Synthesis and Characterization
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5-androstene-3,17-dione (5-AND), androstenolone, equilenin and estrone were purchased from Steraloids Inc. (USA). 15N-Labeled NH4Cl was purchased from Cambridge Isotope Laboratories Inc. (USA). A Superose 12 gel filtration column was purchased from Amersham Bioscience (USA). All chemicals for the buffer solution were purchased from Sigma (USA). All enzymes for DNA manipulation were purchased from Promega (USA). Oligonucleotides were obtained from Genotech Inc. (Korea).
3
Steroid Reference Standards Acquisition
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Standards for the analytes examined in this study (see Table 1 and Fig. 1) were purchased from Steraloids (Newport, RI, USA): 17a-hydroxyprogesterone (17OH-progesterone), 5a-DHP, allopregnanolone (3aDHP), androstenedione, DHEA, d 7 -androstendione (A 4 -d 7 ), estrone, pregnenolone, 5a-pregnan-3b, 20a-diol (3b,20a-dihydroxy-dihydroprogesterone (3b,20a-DHP)), 20a-hydroxy-5a-DHP (20a-hydroxy-dihydroprogesterone (20aDHP)), progesterone and d 9 -progesterone (P 4 -d 9 ). The remaining analytes were purchased from Cerilliant (Round Rock, TX, USA): 19-norandrostenedione, 5a-dihydrotestosterone (DHT), d 3 -testosterone (T-d 3 ), and testosterone. A master mix of all reference standards was prepared and diluted in methanol (10, 1, 0.1 and 0.01 ng/ml). Methanol and water were of HPLC grade and obtained from Burdick and Jackson (Muskegon, MI, USA). Formic acid and methyl-tert buytl ether were of ACS grade and obtained from EMD (Gibbstown, NJ, USA).
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