Forskolin (F6886), NH4Cl (A9434) and leupeptin (L2884) were purchased from Sigma–Aldrich (St. Louis, MO, USA). H89 (#371962) and KT5823 (#420321) were from Calbiochem (San Diego, CA, USA). Anti-perilipin 1 (#9349), anti-HSL (#4107), anti-pHSL (Ser552)(#4139), anti-pHSL (Ser554)(#4137), anti-pHSL (Ser650)(#4126), anti-acetyl-CoA carboxylase (#3676), anti-adiponectin (#2789), anti-CCAAT/enhancer-binding protein α (C/EBPα) (#8178), anti-fatty acid binding protein 4 (FABP4) (#3544) and anti-fatty acid synthase (FAS) (#3180), anti-p62/SQSTM1 (#5114s) antibodies were from Cell Signaling Technology (Beverly, MA, USA). Anti-beclin-1 (NB110-87318), anti-Atg5 (NB110-53818), and anti-LC3 antibody (NB100-2220) was purchased from Novus Biologicals (Littleton, CO, USA). Anti-GAPDH (SC-25778) antibody was from Santa Cruz Biotechnology (Dallas, TX, USA).
Anti atg5 nb110 53818
Manufactured by Novus Biologicals
Sourced in United States
Anti-Atg5 (NB110-53818) is a primary antibody that targets the Atg5 protein. Atg5 is a key component of the autophagy pathway, which is involved in the degradation and recycling of cellular components. This antibody can be used for the detection and analysis of Atg5 in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Anti fatty acid synthase, by Cell Signaling Technology (1 mentions)
Anti hsl, by Cell Signaling Technology (1 mentions)
Anti p hsl, by Cell Signaling Technology (1 mentions)
Anti lc3 antibody nb100 2220, by Novus Biologicals (1 mentions)
Anti beclin 1 nb110 87318, by Novus Biologicals (1 mentions)
Anti gapdh antibody sc 25778, by Santa Cruz Biotechnology (1 mentions)
Anti acetyl coa carboxylase, by Cell Signaling Technology (1 mentions)
Anti adiponectin, by Cell Signaling Technology (1 mentions)
Anti perilipin 1, by Cell Signaling Technology (1 mentions)
Anti ccaat enhancer binding protein α c ebp α, by Cell Signaling Technology (1 mentions)
Anti gfp sc 9996, by Santa Cruz Biotechnology (1 mentions)
Anti lc3 nb100 2220, by Novus Biologicals (1 mentions)
Laemmli sample buffer, by Bio-Rad (1 mentions)
Bradford assay, by Bio-Rad (1 mentions)
Nitrocellulose membrane, by Cytiva (1 mentions)
Enhanced chemiluminescence detection system, by Merck Group (1 mentions)
Gapdh 2118l, by Cell Signaling Technology (1 mentions)
Beclin 1, by Abcam (1 mentions)
3 protocols using anti atg5 nb110 53818
1
Adipocyte Lipolysis and Autophagy Regulation
2
Western Blot Analysis of Melanogenesis Regulators
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All lysates were prepared with 2× Laemmli sample buffer (Bio-Rad, Hercules, CA, USA). Total protein was measured using the Bradford assay (Bio-Rad) according to the manufacturer’s instruction. Samples were separated by SDS-polyacrylamide gel electrophoresis and transferred to PVDF membrane. After blocking with 4% skim milk in tris-buffered saline supplemented with Tween-20, the membrane was incubated with primary antibodies including anti-MITF (MS-771-P1; Neomarkers), anti-TYR (a gift from Amorepacific Research Group), anti-LC3 (NB100-2220), anti-ATG5 (NB110-53818) and anti-actin (NB600-501; NOVUS Biologicals, Littleton, CO, USA), and anti-GFP (sc-9996; Santa Cruz Biotechnology, Dallas, TX, USA). For protein detection, the membranes were incubated with HRP-conjugated secondary antibodies (Pierce, Rockford, IL USA).
3
Western Blot Analysis of Autophagy Proteins
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Cells were lysed on ice for 30 min in lysis buffer containing 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Nonidet P-40, and 0.1% SDS supplemented with protease inhibitors (10 m g/ml leupeptin, 10 m g/ml
pepstatin A, and 10 m g/ml aprotinin). For western analysis, 15μg of sample was resolved by 12% SDS-PAGE and electro-transferred onto nitrocellulose membranes (Whatman, Piscataway, NJ). The primary antibodies used were anti-ULK-1 (NBP2-24738, Novus Biologicals); anti-ATG-5 (NB110-53818, Novus Biologicals); anti-Bcl-2 (2876S, Cell Signaling Technology); LC3 (SAB1306673, Sigma-Aldrich); Beclin-1 (ab55878, Abcam) at a dilution of 1:500. To normalize protein loading, a GAPDH (2118L, Cell Signaling Technology) antibody was used at 1:2,000 dilution. HRP-conjugated secondary antibodies were used at a 1:2,000 dilution. The antigen-antibody complexes were visualized using an enhanced chemiluminescence detection system (Millipore, Billerica, MA) as recommended by the manufacturer.
pepstatin A, and 10 m g/ml aprotinin). For western analysis, 15μg of sample was resolved by 12% SDS-PAGE and electro-transferred onto nitrocellulose membranes (Whatman, Piscataway, NJ). The primary antibodies used were anti-ULK-1 (NBP2-24738, Novus Biologicals); anti-ATG-5 (NB110-53818, Novus Biologicals); anti-Bcl-2 (2876S, Cell Signaling Technology); LC3 (SAB1306673, Sigma-Aldrich); Beclin-1 (ab55878, Abcam) at a dilution of 1:500. To normalize protein loading, a GAPDH (2118L, Cell Signaling Technology) antibody was used at 1:2,000 dilution. HRP-conjugated secondary antibodies were used at a 1:2,000 dilution. The antigen-antibody complexes were visualized using an enhanced chemiluminescence detection system (Millipore, Billerica, MA) as recommended by the manufacturer.
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