Mibolerone (Mb) was from Sigma Aldrich (St. Louis, MO, USA). The antibodies against β-Actin (AC-15), BAD (C-7), BAX (B-9), Bcl-2 (C-2), BID (FL-195), GAPDH (FL-335), and Lamin B (C-20) were from Santa Cruz Biotechnology (Bolivia); OXPHOS and VDAC1 were from Abcam (Cambridge, UK); and AR (D6F11) was from Cell Signaling (Boston, MA, USA).
Mibolerone mb
Manufactured by Merck Group
Sourced in United States
Mibolerone (Mb) is a synthetic anabolic-androgenic steroid. It is commonly used in research and laboratory settings. The core function of Mibolerone is to provide researchers with a potent and selective androgenic agent for various experimental applications.
Automatically generated - may contain errors
2 protocols using mibolerone mb
1
Antibody Reagents for Molecular Analysis
2
Hormone Treatment of Prostate Cancer Cell Lines
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LNCaP or PC3 cell lines were cultured in RPMI 1640 media supplemented with fetal bovine serum (FBS; 11%; Wisent Bio Products, Saint-Jean-Baptiste, QC, Canada), at 37°C with 5% CO2 in plastic culture flasks. Once confluent, 1 ×106 cells were seeded into 6-well plates in the same medium to allow the cells to adhere. Twenty-four hours after plating, the medium was changed to RPMI supplemented with 10% charcoal-dextran stripped FBS (csFBS) and incubated for an additional 24 h. The following day, the medium was changed to fresh RPMI/csFBS for hormone treatment and inhibitor studies. Mibolerone (MB; a synthetic nonmetabolizable androgen) was used in all experiments rather than 5α-dihydrotestosterone (DHT; Sigma, St. Louis, MO, USA) or R1881 allowing for the receptor to maintain its active state for longer periods of time. Hormone treatment of MEF cells was carried out similarly, but in Dulbecco’s modified eagle medium (DMEM; Wisent Bio Products) media.
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