For heart morphometric analyses, each whole heart was embedded in Tissue Tek Optimal Cutting Temperature Compound (OCT, Sakura Finetek, Torrance, CA), and the method used was as described previously [23 (link)]. Briefly, sections (10–12 μM thickness) were treated with hematoxylin and then rinsed with water, Scott's Solution, 95% and 200% ethanol, and eosin, followed by Histo-Clear (National Diagnostics, Atlanta, GA). Measures were taken of whole heart surface area and left ventricle surface area. Area assessments were made with 100x objective (an average of five measures taken per heart was used).
Tissue tek optimal cutting temperature compound oct
Manufactured by Sakura Finetek
Tissue Tek Optimal Cutting Temperature Compound (OCT) is a clear, colorless, water-soluble, glycol-based embedding medium designed for the preparation of frozen tissue sections for histological examination. It is used to embed tissue samples prior to cryosectioning.
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Lab products found in correlation
2 protocols using tissue tek optimal cutting temperature compound oct
1
Heart Morphometric Analysis Protocol
2
Perfusion and Tissue Collection for Mouse Nodose Ganglion and Visual Cortex
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Prior to collection of adult visual cortex and early postnatal nodose ganglion, mice were anesthetized with a Ketamine:Xylazine mixture (100 mg/kg: 10 mg/kg) and transcardially perfused with 4% paraformaldehyde (pH 7.4). Due to the small size of the tissue, a dissecting microscope was used to collect the nodose ganglion from each mouse. All collected tissue was postfixed in 4% paraformaldehyde for 2–3 hours at 4°C, then cryoprotected overnight in 20% sucrose. Tissue samples were embedded in Tissue-Tek Optimal Cutting Temperature Compound (OCT) (Sakura Finetek, Cat. #4583), frozen over dry ice, and stored at −80°C.
20 μM cryosections were collected in six series through the entirety of the nodose ganglion and mounted directly onto slides. For the visual cortex, 20 μM cryosections were collected in the sagittal orientation in 4 series. All slides were stored at −20°C until processing.
20 μM cryosections were collected in six series through the entirety of the nodose ganglion and mounted directly onto slides. For the visual cortex, 20 μM cryosections were collected in the sagittal orientation in 4 series. All slides were stored at −20°C until processing.
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