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Ecm556

Manufactured by Merck Group
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The ECM556 is a laboratory equipment product offered by Merck Group. It is designed for general laboratory use, with a focus on providing a reliable and consistent operating environment. The core function of the ECM556 is to maintain precise temperature and humidity control within a defined workspace. Further details on the intended use or specific applications of this product are not available.

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Lab products found in correlation

Annexin 5 apoptosis detection kit apc, by Thermo Fisher Scientific (2 mentions) Celltiter 96 aqueous one solution assay, by Promega (2 mentions)

2 protocols using ecm556

1

Evaluating Anti-Proliferative and Pro-Apoptotic Effects of PMT

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell proliferation was measured 24 and 48 h of incubation with PMT (10, 25, 50, 75, 100, 150 and 200 μg/mL) using CellTiter 96 Aqueous One solution assay (Promega Corporation, Madison, WI) as described previously [11 (link),12 (link)]. Apoptosis was measured using Annexin V Apoptosis Detection Kit APC (eBioscience, Inc., San Diego, CA) following treatment with PMT (30 h) as per manufacturer’s instructions. Etoposide (Etop) was used as a positive control. Colony forming ability was determined using crystal violet staining. Cell invasion assay was performed according to the manufacturer’s instructions (ECM556, Chemicon, EMD Millipore, Billerica, MA). Immunoblot analysis, Real-Time PCR and transient expression assays were conducted as described previously using either chemiluminescence or Infrared Imaging [11 (link)-13 (link)].
Chakravarthy D., Muñoz A.R., Su A., Hwang R.F., Keppler B.R., Chan D.E., Halff G., Ghosh R, & Kumar A.P. (2018). Palmatine suppresses glutamine-mediated interaction between pancreatic cancer and stellate cells through simultaneous inhibition of survivin and COL1A1. Cancer letters, 419, 103-115.
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2

Evaluating Anti-Proliferative and Pro-Apoptotic Effects of PMT

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell proliferation was measured 24 and 48 h of incubation with PMT (10, 25, 50, 75, 100, 150 and 200 μg/mL) using CellTiter 96 Aqueous One solution assay (Promega Corporation, Madison, WI) as described previously [11 (link),12 (link)]. Apoptosis was measured using Annexin V Apoptosis Detection Kit APC (eBioscience, Inc., San Diego, CA) following treatment with PMT (30 h) as per manufacturer’s instructions. Etoposide (Etop) was used as a positive control. Colony forming ability was determined using crystal violet staining. Cell invasion assay was performed according to the manufacturer’s instructions (ECM556, Chemicon, EMD Millipore, Billerica, MA). Immunoblot analysis, Real-Time PCR and transient expression assays were conducted as described previously using either chemiluminescence or Infrared Imaging [11 (link)-13 (link)].
Chakravarthy D., Muñoz A.R., Su A., Hwang R.F., Keppler B.R., Chan D.E., Halff G., Ghosh R, & Kumar A.P. (2018). Palmatine suppresses glutamine-mediated interaction between pancreatic cancer and stellate cells through simultaneous inhibition of survivin and COL1A1. Cancer letters, 419, 103-115.
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