Cells grown and treated as indicated were collected and total protein was extracted. The following primary antibodies were used: rabbit monoclonal anti-α-actin (Abcam), monoclonal anti-COL1A1 (Millipore), rabbit monoclonal anti-phosphorylated SMAD2, rabbit monoclonal anti-phosphorylated SMAD3, rabbit monoclonal anti- SMAD2/3, rabbit monoclonal anti-ERK1/2, or rabbit monoclonal anti-phosphorylated ERK1/2, rabbit monoclonal anti-Akt, rabbit monoclonal anti-phosphorylated Akt, rabbit monoclonal anti-STAT3, or rabbit monoclonal anti-phosphorylated STAT3 (all from Cell Signaling Technology, Inc.). Horseradish peroxidase-conjugated goat-anti-rabbit antibody (Thermo Scientific) was used as a secondary antibody. The control for equal protein loading was assessed with an anti-β-actin antibody (Cell Signaling Technology, Inc.).
Rabbit monoclonal anti phosphorylated akt
Manufactured by Cell Signaling Technology
Rabbit monoclonal anti-phosphorylated Akt is a laboratory reagent used for the detection and quantification of phosphorylated Akt protein. It is a highly specific and sensitive tool for researchers studying cell signaling pathways involving Akt activation.
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Lab products found in correlation
Rabbit monoclonal anti smad2 3, by Cell Signaling Technology (1 mentions)
Rabbit monoclonal anti erk1 2, by Cell Signaling Technology (1 mentions)
Rabbit monoclonal anti akt, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase conjugated goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions)
Anti β actin antibody, by Cell Signaling Technology (1 mentions)
Rabbit monoclonal anti stat3, by Cell Signaling Technology (1 mentions)
Rabbit monoclonal anti phosphorylated stat3, by Cell Signaling Technology (1 mentions)
Mouse monoclonal anti e cadherin, by BD (1 mentions)
Rabbit polyclonal anti akt, by Cell Signaling Technology (1 mentions)
Goat polyclonal anti actin, by Santa Cruz Biotechnology (1 mentions)
Polyclonal rabbit anti trip 1, by Abcam (1 mentions)
2 protocols using rabbit monoclonal anti phosphorylated akt
1
Protein Expression Analysis of Cellular Pathways
2
Western Blot Analysis of Lung and Cell Lysates
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Total lung or cell lysates were prepared using previously described methods (Navarro et al. 2009 ; Perez et al. 2011 ; Nyp et al. 2014 ). Western blots were developed using ECL (chemiluminescence) with the following antibodies: polyclonal rabbit anti‐TRIP‐1 (1:1000, Abcam); mouse monoclonal antismooth muscle actin (1:1000) and tubulin (1:10,000) (Sigma‐Aldrich); rabbit monoclonal antiphosphorylated Akt (1:1000) and rabbit polyclonal anti‐Akt (1:1000) (Cell Signaling); mouse monoclonal anti‐E‐cadherin (1:1000, BD Biosciences); and goat polyclonal antiactin (1:2000, Santa Cruz Biotechnologies).
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