Genomic g 100 columns

Two hundred microliters of each previously processed clinical sample or yeast suspension were used for DNA extraction and purification. The QIAamp^® DNA Mini kit (Qiagen, Hildenberg, Germany) was used with some modifications: the initial incubation with lysis buffer was performed at 65 °C for one hour, followed by AL buffer incubation at 90 °C for 10 min and additional incubation with recombinant lyticase (1 UI/μL) at 37 °C for 45 min. For filamentous fungal isolates, DNA extraction was performed using the phenol–chloroform method or a commercial kit with Genomic G-100 columns (Qiagen Inc., CA).³¹ DNA extraction from whole blood was performed using a protocol described by Einsele et al.,³² (link) with some modifications. The relative concentrations of DNA extracted were determined using a NanoDrop ND2000 (Thermo Scientific).

Two hundred microliters of each previously processed clinical sample or yeast suspension were used for DNA extraction and purification. The QIAamp^® DNA Mini kit (Qiagen, Hildenberg, Germany) was used with some modifications: the initial incubation with lysis buffer was performed at 65° C for one hour, followed by AL buffer incubation at 90 °C for 10 min and an additional incubation with recombinant lyticase (1UI/μl) at 37° C for 45 min. For filamentous fungal isolates, DNA extraction was performed using the phenol-chloroform method or a commercial kit with Genomic G-100 columns (Qiagen Inc., CA) (Sambrook et al., 2001).³³ DNA extraction from whole blood was performed using a protocol described by Einsele et al. (1997)³⁴ (link) with some modifications. The relative concentrations of DNA extracted were determined using a NanoDrop ND2000 (Thermo Scientific).