To assess the role of CD4 T cells in programming CD8 T cell memory, mice were depleted of CD4 T cells by administration of 200-250 μg of anti-CD4 antibodies (Bio X Cell, Clone: GK1.5) intraperotoneally and intranasally on days −1, 0 and 1 relative to vaccinations. To assess the role of CD4 T cells and CD8 T cells in protective immunity, mice were administered with 200 μg of anti-CD4 (Bio X Cell, Clone: GK1.5) or CD8 T cells (Bio X Cell; Clone 2.43) intravenously and intranasally at days −5. −3, −1 and 1 relative to challenge with influenza A virus. To determine whether IL-17A was involved in vaccine-induced protection against influenza virus, vaccinated mice were treated with 200 μg of isotype control antibodies or anti-IL-17A antibodies (Bio X Cell; intravenously and intranasally) at days -3, −1 and 1, 3 amd 5 relative to challenge with influenza A virus.
Anti il 17a antibodies
Manufactured by BioXCell
Anti-IL-17A antibodies are laboratory reagents that can be used to detect and quantify the presence of the cytokine Interleukin-17A (IL-17A) in biological samples. IL-17A is a pro-inflammatory cytokine involved in various immune processes. These antibodies can be utilized in research applications such as ELISA, Western blotting, and immunohistochemistry to study the role of IL-17A in different biological systems and disease models.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using anti il 17a antibodies
1
Elucidating CD4 and CD8 T Cell Roles in Influenza Immunity
2
Dissecting CD4 and CD8 T Cell Roles in Influenza Immunity
3
Acute Inflammatory Arthritis in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Acute inflammatory arthritis was induced in knee joints of C57BL/6N mice as previously described20 (link) by intra-articular injection with 200 µg of mBSA (Sigma A-1009), followed by daily injections on days 0–2 with 250 ng of human IL-1β (BioLegend). At time of sacrifice, knee joints were isolated for histological analysis and qPCR. Draining popliteal and inguinal lymph nodes were isolated for flow cytometry analysis. The treatment with the TGF-βRI inhibitor SB-505124 (Sigma-Aldrich) was applied locally starting two hours before the induction of arthritis, and mice were injected intra-articularly on four consecutive days with either saline, 20% DMSO as vehicle control, or 75 nmol SB-505124 (Sigma-Aldrich) in 20% DMSO with an injection volume of 6 µl per joint. Anti-IL-17A antibodies (BioXCell) were used as positive control, administered as 50 µg antibody/mouse intra-peritoneally injected every other day (n = 8 mice/group). Mice were sacrificed by cervical dislocation four days after arthritis induction, and knee joints were subsequently isolated for histological analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!