The number of leukocytes adherent to the microvasculature was determined at 3 months of diabetes. After cardiac catheterization, anesthetized mice (100 mg/mL ketaset to 100 mg/mL xylazine ratio of 5:1) were exsanguinated by perfusion with PBS. Fluorescein-coupled concanavalin A lectin (20 µg/mL in PBS; Vector Laboratories, Burlingame, CA, USA) was then infused as previously described.26 (link) Flat-mounted retinas were viewed via fluorescence microscopy, and brightly fluorescent leukocytes were counted in the entire retina.
Fluorescein coupled concanavalin a lectin
Manufactured by Vector Laboratories
Sourced in United States
Fluorescein-coupled concanavalin A lectin is a protein that binds to carbohydrates and is labeled with the fluorescent dye fluorescein. It is used in various biological applications to detect and study carbohydrate-containing molecules and structures.
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Lab products found in correlation
2 protocols using fluorescein coupled concanavalin a lectin
1
Quantifying Leukocyte Adhesion in Diabetic Retina
2
Fluorescein-coupled Concanavalin A Lectin Retinal Imaging
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