Trypsin enzyme gold ms grade

5-aminolevulinic acid (5-ALA) was from Medac (Wedel, Germany). Iodoacetamide (IAA), d,l-dithiothreitol (DTT), Ammonium bicarbonate (AMBIC), bovine serum albumin and acetone were from Sigma-Aldrich (St. Louis, MO, USA). Water, acetonitrile (ACN), formic acid (FA) were from Merck (Darmstadt, Germany). All organic solvents were of LC-MS grade. Trypsin enzyme (Gold MS Grade) was from Promega (Madison, WI, USA).

Human Lactoferrin (LF) recombinant, expressed in rice, Iron saturated, ≥ 90% (SDS-PAGE), Human Lactoferrin partially iron saturated (LF_p iron saturation ~ 0.07%) recombinant, expressed in rice, ≥ 90% (SDS-PAGE), dithiothreitol (DTT), urea, ethylendiamminotetraacetic acid (EDTA), 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB), 1-chloro-2,4-dinitrobenzene (CDNB), 4-chloro-7-nitrobenzofurazane (NBD-Cl), N-acetyl-L-tryptophanamide (NATA), bromopyruvic acid (BrP), cystamine, cystine, homocystine, oxidized glutathione (GSSG), and ammonium bicarbonate (AMBIC) was from Sigma-Aldrich (St. Louis, MO, USA). Water and formic acid (FA), LC–MS grade, were from Merck (Darmstadt, Germany). Trypsin enzyme (Gold MS Grade) was from Promega (Madison, WI, USA). Acetonitrile (ACN) (LC–MS grade) was from Merck (Darmstadt, Germany).

5-aminolevulinic acid (5-ALA) was from Medac (Wedel, Germany). Iodoacetamide (IAA), D,L-dithiothreitol (DTT), Ammonium bicarbonate (AMBIC), bovine serum albumin and acetone were from Sigma-Aldrich (St. Louis, MO, USA). Water, acetonitrile (ACN), formic acid (FA) were from Merck (Darmstadt, Germany). All organic solvents were of LC-MS grade. Trypsin enzyme (Gold MS Grade) was from Promega (Madison, WI, USA).