Dad 1290 detector

Manufactured by Agilent Technologies

The DAD 1290 detector is a high-performance diode array detector (DAD) designed for liquid chromatography (LC) applications. The core function of the DAD 1290 is to detect and quantify compounds in liquid samples by measuring the absorbance of light at multiple wavelengths simultaneously.

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Lab products found in correlation

Ultra performance liquid chromatography (uplc), by Agilent Technologies (2 mentions)

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DAD detector (45 citations)

2 protocols using dad 1290 detector

Phenolic Compounds Analysis by UPLC

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The procedure was based on the method published by Kobus et al. [12 (link)]. Phenolic acids and flavonols were analyzed using Agilent UPLC equipped with a Bin Pump Infinity DAD 1290 detector (for phenolics acids λ = 260 nm and 310 nm and for flavonols λ = 370 nm). Separated compounds of phenolic acids and individual flavonols were determined as chlorogenic acid, ferulic acid, vanillic acid, gallic acid, o-coumaric acid, p-coumaric acid, cinnamic acid, syringic acid, p-hydroxybenzoic acid, caffeic acid, catechin, epicatechin, quercetin, rutin, and kaempferitrin. Compounds were identified using the standards dissolved in methanol.
The single-reference method was applied to determine a relationship between peak area and the concentration of the analyzed standard.

Kobus-Cisowska J., Szymanowska-Powałowska D., Szczepaniak O., Kmiecik D., Przeor M., Gramza-Michałowska A., Cielecka-Piontek J., Smuga-Kogut M, & Szulc P. (2019). Composition and In Vitro Effects of Cultivars of Humulus lupulus L. Hops on Cholinesterase Activity and Microbial Growth. Nutrients, 11(6), 1377.

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Phenolic Acid Analysis via UPLC

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The procedure was based on the methodology published by Kobus et al. (2009) . The phenolic acids were analyzed as a percentage composition using Agilent UPLC equipped with a Bin Pump Infi nity DAD 1290 detector (λ = 260 nm and 310 nm). A non-linear concentration gradient was used to analyze the dry extract. The gradient consisted of H 3 PO 4 buff er (solvent A), pH = 2.7, which was adjusted using 1:1 v/v acetonitrile-water (solvent B). The gradient profi le decreased from 95% of solvent A at 1 min to 50% of B at 52 min and the run time was 58 min. The volume of samples after fi ltration (PTFE fi lter; 0.45 μm) was 10 μl. Phenolic acids were identifi ed using the standards dissolved in methanol (caff eic, chlorogenic, ferulic, p-coumaric and sinapic acids).

Kulczyński B., Kobus-Cisowska J., Kmiecik D., Gramza-Michałowska A., Golczak D, & Korczak J. (2016). Antiradical capacity and polyphenol composition of asparagus spears varieties cultivated under different sunlight conditions. Acta scientiarum polonorum. Technologia alimentaria, 15(3).

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