The cells isolated from digested lungs were stained with biotin-conjugated antibody mixtures for lineage markers (CD4, CD5, CD8, CD11c, CD11b, CD19, NK1.1, Gr-1, TER119, FcεRI, and B220), Pacific blue-conjugated anti-Sca-1, PECy7-conjugated c-Kit (CD117), APC-conjugated anti-IL-7Rα (CD127), FITC conjugated anti-T1/ST2, APC-Cy7-conjugated anti-CD25, and PE conjugated anti-streptavidin, and analyzed using a MACSQuant Analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany). Lin−Sca+c-Kit+IL-7R+CD25+ST2dim cells were identified as lung ILC2s [21 (link), 22 (link)]. The data were analyzed using FlowJo (TreeStar, Ashland, OR, USA). APC-Cy7-conjugated anti-CD25, Pacific blue-conjugated anti-Sca-1, biotin-conjugated anti-CD4, anti-CD5, anti-CD8, anti-CD11b, anti-NK1.1, anti-Gr-1, anti-TER119, and anti-B220, and PE-conjugated anti-streptavidin antibodies were obtained from BD Biosciences (Franklin Lakes, NJ, USA). FITC-conjugated anti-T1/ST2 was from MD Bioscience (St Paul, MN, USA). APC-conjugated anti-IL-7Rα and biotin-conjugated anti-FcεRI antibodies were from BioLegend (San Diego, CA, USA). PECy7-conjugated c-Kit was from eBioscience (La Jolla, CA, USA). Biotin-conjugated anti-CD11c and anti-CD19 were from TONBO Biosciences (San Diego, CA, USA).
Biotin conjugated anti cd4
Manufactured by BD
Sourced in United States
Biotin-conjugated anti-CD4 is a laboratory reagent used for the detection and identification of CD4-positive cells. It consists of a monoclonal antibody specific to the CD4 antigen, conjugated with biotin. This product is intended for research use only and its core function is to enable the identification and isolation of CD4-positive cells in various experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Apc conjugated anti il 7rα, by Miltenyi Biotec (2 mentions)
Fitc conjugated anti t1 st2, by Miltenyi Biotec (2 mentions)
Pe conjugated anti streptavidin, by BD (2 mentions)
Apc conjugated anti il 7rα, by BioLegend (2 mentions)
Biotin conjugated anti fcεri antibodies, by BioLegend (2 mentions)
Pecy7 conjugated c kit, by Thermo Fisher Scientific (2 mentions)
Anti cd8, by BD (2 mentions)
Anti ter119, by BD (2 mentions)
Anti gr 1, by BD (2 mentions)
Anti b220, by BD (2 mentions)
Anti cd5, by BD (2 mentions)
Anti nk1, by BD (2 mentions)
Apc cy7 conjugated anti cd25, by BD (2 mentions)
Anti cd11b, by BD (2 mentions)
Macsquant analyzer, by Miltenyi Biotec (2 mentions)
Anti cd19, by Cytek Biosciences (1 mentions)
2 protocols using biotin conjugated anti cd4
1
Lung Innate Lymphoid Cell Type 2 Isolation
2
Identification and Analysis of Lung ILC2s
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Analyses of ILC2s. The cells isolated from digested lungs were stained with biotin-conjugated antibody mixtures for lineage markers (CD4, CD5, CD8, CD11c, CD11b, CD19, NK1.1, Gr-1, TER119, FcεRI, and B220), Paci c blue-conjugated anti-Sca-1, PECy7-conjugated c-Kit (CD117), APC-conjugated anti-IL-7Rα (CD127), FITC conjugated anti-T1/ST2, APC-Cy7-conjugated anti-CD25, and PE conjugated antistreptavidin, and analyzed using a MACSQuant Analyzer (Miltenyi Biotec, Bergisch Gladbach, Germany).
Lin -Sca + c-Kit + IL-7R + CD25 + ST2 dim cells were identi ed as lung ILC2s [21] [22] . The data were analyzed using FlowJo (TreeStar, Ashland, OR, USA). APC-Cy7-conjugated anti-CD25, Paci c blue-conjugated anti-Sca-1, biotin-conjugated anti-CD4, anti-CD5, anti-CD8, anti-CD11b, anti-NK1.1, anti-Gr-1, anti-TER119, and anti-B220, and PE-conjugated anti-streptavidin antibodies were obtained from BD Biosciences (Franklin Lakes, NJ, USA). FITC-conjugated anti-T1/ST2 was from MD Bioscience (St Paul, MN, USA). APCconjugated anti-IL-7Rα and biotin-conjugated anti-FcεRI antibodies were from BioLegend (San Diego, CA, USA). PECy7-conjugated c-Kit was from eBioscience (La Jolla, CA, USA). Biotin-conjugated anti-CD11c and anti-CD19 were from TONBO Biosciences (San Diego, CA, USA).
Lin -Sca + c-Kit + IL-7R + CD25 + ST2 dim cells were identi ed as lung ILC2s [21] [22] . The data were analyzed using FlowJo (TreeStar, Ashland, OR, USA). APC-Cy7-conjugated anti-CD25, Paci c blue-conjugated anti-Sca-1, biotin-conjugated anti-CD4, anti-CD5, anti-CD8, anti-CD11b, anti-NK1.1, anti-Gr-1, anti-TER119, and anti-B220, and PE-conjugated anti-streptavidin antibodies were obtained from BD Biosciences (Franklin Lakes, NJ, USA). FITC-conjugated anti-T1/ST2 was from MD Bioscience (St Paul, MN, USA). APCconjugated anti-IL-7Rα and biotin-conjugated anti-FcεRI antibodies were from BioLegend (San Diego, CA, USA). PECy7-conjugated c-Kit was from eBioscience (La Jolla, CA, USA). Biotin-conjugated anti-CD11c and anti-CD19 were from TONBO Biosciences (San Diego, CA, USA).
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