Transwell migration was performed as previously described 70 (link). Invasion assays were performed with the BD Biocoat growth factor reduced Matrigel invasion chambers kit in 24-well tissue culture plates. Five-hundred microliter breast cancer cell suspensions were added to the upper side of the chambers at a density of 1 × 105 cells/insert and 750 µl CM was added to the lower wells. The number of migrated cells in 5 fields of view per insert was counted under a light microscope at magnification 10 X. Experiments were carried out in triplicate.
Biocoat growth factor reduced matrigel invasion chambers kit
Manufactured by BD
The BD Biocoat growth factor reduced Matrigel invasion chambers kit is a laboratory product designed to assess cell invasion through a basement membrane matrix. The kit consists of pre-coated cell culture inserts that can be used in a standard cell culture system.
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Lab products found in correlation
2 protocols using biocoat growth factor reduced matrigel invasion chambers kit
1
Transwell Invasion Assay for Breast Cancer Cells
2
Transwell Invasion Assay for Breast Cancer Cells
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