Anti claudin 1

Manufactured by Bioss Antibodies

Sourced in China, United States

Anti-claudin-1 is a laboratory reagent that can be used to detect the presence of the claudin-1 protein in biological samples. Claudin-1 is a tight junction protein that plays a role in the regulation of cell-cell adhesion. The anti-claudin-1 antibody can be used in various research applications, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and localization of claudin-1 in different cell types and tissues.

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Lab products found in correlation

Anti occludin occ, by Bioss Antibodies (2 mentions) Anti tubulin antibodies, by Bioss Antibodies (2 mentions) Anti zo 1, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Claudin-1 (10 citations) Rabbit anti-Claudin 1 (3 citations)

2 protocols using anti claudin 1

Western Blot Analysis of Jejunum Tight Junctions

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Expression of tight junction proteins of jejunum was determined by WB analysis, which was conducted as previously described (Meng (link)
et al., 2017 (link)). Primary antibodies used include anti-zonula occludens 1 (ZO-1) (1:2,000, Santa Cruz, USA), anti-occludin (OCC) (1:500, Bioss, China), anti-claudin-1 (1:500, Bioss, China) and anti-tubulin antibodies (1:5,000, Bioss, China). Finally, chemiluminescence detection was performed with western ECL reagent on a FluorChem M Fluorescent Imaging System (ProteinSimple, Santa Clara, CA, USA). Densitometry analysis was perform using Image J software (NIH, Bethesda, MD, USA).

Song M., Zhang F., Chen L., Yang Q., Su H., Yang X., He H., Ling M., Zheng J., Duan C., Lai X., Pan M., Zhu X., Wang L., Gao P., Shu G., Jiang Q, & Wang S. (2021). Dietary chenodeoxycholic acid improves growth performance and intestinal health by altering serum metabolic profiles and gut bacteria in weaned piglets. Animal Nutrition, 7(2), 365-375.

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Western Blot Analysis of Tight Junction Proteins

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Expression of tight junction (TJ) proteins in jejunum was determined by WB analysis which was conducted as previously described [25 (link)]. Proteins were detected by primary antibodies: anti-Tubulin antibodies (1:5000, Bioss, China), anti-Occludin (OCC) (1:500, Bioss, China), anti-ZO-1 (1:2000, Santa Cruz, USA) and anti-Claudin-1 (1:500, Bioss, China), and then incubated with the secondary antibodies (1:2000, Bioss, China). Finally, chemiluminescence detection was performed with western ECL reagent and band densities were quantified using a FluorChem M Fluorescent Imaging System (ProteinSimple, Santa Clara, CA, USA). The intensity of bands was analyzed and quantified by Image J Sofware (NIH, Bethesda, MD, USA).

Song M., Zhang F., Fu Y., Yi X., Feng S., Liu Z., Deng D., Yang Q., Yu M., Zhu C., Zhu X., Wang L., Gao P., Shu G., Ma X., Jiang Q, & Wang S. (2022). Tauroursodeoxycholic acid (TUDCA) improves intestinal barrier function associated with TGR5-MLCK pathway and the alteration of serum metabolites and gut bacteria in weaned piglets. Journal of Animal Science and Biotechnology, 13, 73.

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