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2 protocols using adenosine 5 γ 32p triphosphate γ 32p atp

1

Characterization of PPM1D Protein Interactions

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Anti-p53, anti-PPM1D, anti-GST and anti-histidine were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-mouse p53 (1C12) was purchased from Cell Signaling Technology (Beverly, MA). Anti-HA was purchased from Covance (San Diego, CA). Anti-actin, proteinase inhibitor cocktail, RNase A, protein A/G beads, PPM1D inhibitor (CCT007093) were purchased from Sigma (St. Louis, MO). Anti-RBM38 antibody was made as previously described 1 (link). The Iscript cDNA synthesis kit was purchased from Bio-Rad Laboratories (Irvine, CA). 35S-methionine/cysteine and adenosine-5'-[γ-32P] Triphosphate ([γ-32P] ATP) were purchased from PerkinElmer (Waltham, MA). The Ni-NTA agarose beads were purchased from Biontex (Germany). The glutathione sepharose beads were purchased from Macherey-Nagel (Germany). The EST clone (clone ID 5295521), which contains a full length human PPM1D cDNA, was purchased from OpenBiosystems (Huntsville, AL).
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2

Characterization of PPM1D Protein Interactions

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-p53, anti-PPM1D, anti-GST and anti-histidine were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-mouse p53 (1C12) was purchased from Cell Signaling Technology (Beverly, MA). Anti-HA was purchased from Covance (San Diego, CA). Anti-actin, proteinase inhibitor cocktail, RNase A, protein A/G beads, PPM1D inhibitor (CCT007093) were purchased from Sigma (St. Louis, MO). Anti-RBM38 antibody was made as previously described 1 (link). The Iscript cDNA synthesis kit was purchased from Bio-Rad Laboratories (Irvine, CA). 35S-methionine/cysteine and adenosine-5'-[γ-32P] Triphosphate ([γ-32P] ATP) were purchased from PerkinElmer (Waltham, MA). The Ni-NTA agarose beads were purchased from Biontex (Germany). The glutathione sepharose beads were purchased from Macherey-Nagel (Germany). The EST clone (clone ID 5295521), which contains a full length human PPM1D cDNA, was purchased from OpenBiosystems (Huntsville, AL).
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