Malondialdehyde mda assay

Cell Bank of the Chinese Academy of Sciences provided the H9c2 cell line (#GNR5). All the cell growth medium used in this study was obtained from HyClone (#SH30023.01B). Foetal bovine serum (FBS) was bought from Tianhang Biotechnology Company (#11011‐8611). Lipopolysaccharide (LPS) was purchased from Beyotime Biotechnology (#S1732), Cell counting assay Kit 8 (CCK‐8) was obtained from Dojindo (#CK04). The commercial ELISA assay kits were purchased from Elabscience Biotechnology and used to detected the levels of the inflammatory cytokine such as IL‐6, TNF‐α. The levels of oxidative stress were evaluated by Superoxide dismutase (SOD, #A001‐3), Catalase (CAT, #A007‐1–1) Lactate dehydrogenase (LDH), Creatine kinase (CK), CK isoenzymes (CK‐MB), and Malondialdehyde (MDA) assay commercial kits, all of above kits were obtained from Jiancheng Bioengineering Institute. Cardiac troponin I (cTnT) assay kit from Roche Diagnostics GmbH. Flow cytometry apoptosis kit was purchased from BD Biosciences (#550474); TUNEL assay kit purchased from Roche (#11684817910). Dihydroethidium (DHE) was from Beyotime Biotechnology (#S0063). The primary antibodies of GAPDH, Bax and Bcl‐2 were purchased from Sigma‐Aldrich. The primary antibodies of PCAF, α‐actinin, NF‐κB p65and phosphorylation of NF‐κB p65 Ser536 were purchased from Cell Signaling Technology.