Human tnf α platinum elisa kit

Serum TNF-α concentrations were measured using a commercial available high-sensitivity enzyme-linked immunosorbent assay (ELISA) kit (R&D Systems, Minneapolis, MN, USA). The lower limit of detection is 0.106 pg/mL, and the intra- and inter-assay coefficients of variation of the assay were 3.1 and 7.2 %, respectively.
The urinary TNF-α values were below or just at the threshold of detection using a human TNF-α Platinum ELISA kit (eBioscience, San Diego, CA, USA); therefore, the values were measured again using a high-sensitivity ELISA kit (R&D Systems).
Serum hs-CRP levels were measured using a latex agglutination method and an automatic analyzer (Hitachi, Tokyo, Japan).

TNF-α levels in the supernatant of PC-3 cell culture were detected using human TNF-α Platinum ELISA kit (eBioscience, Vienna, Austria) according to the manufacturers’ instructions. A standard curve was prepared from human TNF-α standard dilutions and human TNF-α sample concentration was determined. Values were normalized to total cell number at the end of experiment.