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Anti total stat6

Manufactured by Abcam

Anti-total-STAT6 is a primary antibody that can be used to detect STAT6 in various applications. STAT6 is a member of the STAT family of transcription factors, which play important roles in cellular signaling pathways. This antibody can be used to measure the total STAT6 protein levels in samples.

Automatically generated - may contain errors

2 protocols using anti total stat6

1

STAT6 and GATA3 Signaling in Intestinal Immunity

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SM-dissected areas of ilea and isolated MLNs were lysed and centrifuged. Supernatants were standardized through the Bradford assay (BioRad, Hercules, CA), and Western blot was completed on 0.45-μm pore-size nitrocellulose membranes (Thermo Scientific, Rockford, IL) and blotted with anti-phopsho-STAT6, anti-total-STAT6, anti-GATA3, anti-IL-13, and anti-actin (AbCam, Cambridge, MA). Immunoreactive bands were revealed with an enhanced chemiluminescent substrate for detection of HRP (Thermo Scientific, Waltham, MA), and blots were exposed to blue basic autorad films (Bioexpress, Kaysville, UT).
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2

STAT6 and GATA3 Signaling in Intestinal Immunity

Check if the same lab product or an alternative is used in the 5 most similar protocols
SM-dissected areas of ilea and isolated MLNs were lysed and centrifuged. Supernatants were standardized through the Bradford assay (BioRad, Hercules, CA), and Western blot was completed on 0.45-μm pore-size nitrocellulose membranes (Thermo Scientific, Rockford, IL) and blotted with anti-phopsho-STAT6, anti-total-STAT6, anti-GATA3, anti-IL-13, and anti-actin (AbCam, Cambridge, MA). Immunoreactive bands were revealed with an enhanced chemiluminescent substrate for detection of HRP (Thermo Scientific, Waltham, MA), and blots were exposed to blue basic autorad films (Bioexpress, Kaysville, UT).
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